Protein Variants | Comment | Organism |
---|---|---|
D157N | inactive mutant | Caenorhabditis elegans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microtubule | acetylation of alpha-tubulin K40 by alphaTAT1 inside the microtubule lumen | Caenorhabditis elegans | 5874 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + [alpha-tubulin]-L-lysine | Caenorhabditis elegans | K40 of alpha-tubulin is the sole site of acetylation by alphaTAT1, alphaTAT1 specifically acetylates K40 of alpha-tubulin and prefers microtubules over free tubulin, overview | CoA + [alpha-tubulin]-N6-acetyl-L-lysine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
- |
- |
no activity in Potorous tridactylis | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | robust alpha-tubulin K40 acetylation in the touch receptor neurons | Caenorhabditis elegans | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + [alpha-tubulin]-L-lysine | K40 of alpha-tubulin is the sole site of acetylation by alphaTAT1, alphaTAT1 specifically acetylates K40 of alpha-tubulin and prefers microtubules over free tubulin, overview | Caenorhabditis elegans | CoA + [alpha-tubulin]-N6-acetyl-L-lysine | - |
? | |
acetyl-CoA + [alpha-tubulin]-L-lysine | alphaTAT1 acetylates tubulin through its GNAT domain in vitro. K40 of alpha-tubulin is the sole site of acetylation by alphaTAT1. alphaTAT1 displays a greater catalytic efficiency for taxol-stabilized microtubules than for free tubulin | Caenorhabditis elegans | CoA + [alpha-tubulin]-N6-acetyl-L-lysine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-tubulin K40 acetyltransferase | - |
Caenorhabditis elegans |
alphaTAT1 | - |
Caenorhabditis elegans |
alphaTAT2 | - |
Caenorhabditis elegans |
Mec-17 | - |
Caenorhabditis elegans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Caenorhabditis elegans |
General Information | Comment | Organism |
---|---|---|
evolution | broad phylogenetic distribution of alphaTAT1 | Caenorhabditis elegans |
malfunction | in animals lacking MEC-17, alphaTAT-2, and the sole Caenorhabditis elegans K40 alpha-tubulin MEC-12, touch sensation can be restored by expression of an acetyl-mimic MEC-12 K40Q. Transient overexpression of alphaTAT1 in PtK2 cells is sufficient to acetylate nearly all microtubules, whereas catalytically inactive alphaTAT1 D157N or the HAT Elp3 fail to detectably elevate alpha-tubulin K40 acetylation | Caenorhabditis elegans |
physiological function | alphaTAT1 is the major and possibly the sole alpha-tubulin K40 acetyltransferase in mammals and nematodes, and tubulin acetylation plays a conserved role in several microtubule-based processes. The worm incorporates acetylated alpha-tubulin into their microtubules. alphaTAT1 is required for the acetylation of axonemal microtubules. In Caenorhabditis elegans, microtubule acetylation is most prominent in touch receptor neurons and MEC-17, a homologue of alphaTAT1, and its paralogue alphaTAT-2 are required for alpha-tubulin acetylation and for two distinct types of touch sensation. Worm mechanosensation requires K40 alpha-tubulin acetylation | Caenorhabditis elegans |