Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C41(DE3) cells | Danio rerio |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-arginine | partially inhibited by L-arginin | Danio rerio |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
acetyl-CoA | apparent value, recombinant mature enzyme, pH and temperature not specified in the publication | Danio rerio | |
0.23 | - |
acetyl-CoA | apparent value, recombinant catalytic domain, pH and temperature not specified in the publication | Danio rerio | |
1.12 | - |
L-glutamate | apparent value, recombinant catalytic domain, pH and temperature not specified in the publication | Danio rerio | |
1.13 | - |
L-glutamate | apparent value, recombinant mature enzyme, pH and temperature not specified in the publication | Danio rerio |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
4 * 52000, catalytic domain, SDS-PAGE | Danio rerio |
52752 | - |
4 * 52752, catalytic domain, calculated from amino acid sequence | Danio rerio |
55000 | - |
4 * 55000, mature enzyme, SDS-PAGE | Danio rerio |
55498 | - |
4 * 55498, mature enzyme, calculated from amino acid sequence | Danio rerio |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-glutamate | Danio rerio | - |
CoA + N-acetyl-L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap nickel affinity column chromatography | Danio rerio |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-glutamate | - |
Danio rerio | CoA + N-acetyl-L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 52000, catalytic domain, SDS-PAGE | Danio rerio |
homotetramer | 4 * 52752, catalytic domain, calculated from amino acid sequence | Danio rerio |
homotetramer | 4 * 55000, mature enzyme, SDS-PAGE | Danio rerio |
homotetramer | 4 * 55498, mature enzyme, calculated from amino acid sequence | Danio rerio |
Synonyms | Comment | Organism |
---|---|---|
N-acetylglutamate synthase | - |
Danio rerio |
NAGS | - |
Danio rerio |
Organism | Comment | Expression |
---|---|---|
Danio rerio | the enzyme is expressed between 24 and 48 h post fertilization | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme plays a role in citrulline and/or arginine biosynthesis during the first day of development and in ammonia detoxification thereafter | Danio rerio |