Cloned (Comment) | Organism |
---|---|
gene PA1377, cloning in Escherichia coli strain JM109, expression in Escherichia coli strain BL21(DE3) | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 0.001 ml protein solution containing 13 mg/ml protein with 0.001 ml of reservoir solution containing 0.1 M Tris-HCl, pH 6.5, 1.6 M ammonium sulfate, and 0.1% w/v sodium azide, 18°C, 1-2 days, cryoprotection in 0.1 M Tris-HCl, pH 6.5, 1.6 M ammonium sulfate, 30% v/v glycerol and 0.1% w/v sodium azide, X-ray diffraction structure determination and analysis at 2.25 A resolution | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9I3W7 | gene PA1377 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + L-methionine | pitax is an acetyltransferase and weakly catalyses the acetylation of l-methionine and l-methionine sulfoxide. Pitax belongs to the GCN5-related N-acetyltransferase family and contains all four sequence motifs conserved among family members. The beta4-strand structure in one of these motifs is disrupted, which is believed to affect binding of the substrate that accepts the acetyl group from acetyl-CoA | Pseudomonas aeruginosa | CoA + N-acetyl-L-methionine | - |
? | |
acetyl-CoA + L-methionine sulfoxide | pitax is an acetyltransferase and weakly catalyses the acetylation of l-methionine and l-methionine sulfoxide. Pitax belongs to the GCN5-related N-acetyltransferase family and contains all four sequence motifs conserved among family members. The beta4-strand structure in one of these motifs is disrupted, which is believed to affect binding of the substrate that accepts the acetyl group from acetyl-CoA | Pseudomonas aeruginosa | CoA + N-acetyl-L-methionine sulfoxide | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | pitax is an alpha/beta-fold protein with an overall structure of two regions of antiparallel beta-sheet, each flanked by alpha-helices | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
More | pitax belongs to the GCN5-related N-acetyltransferase family | Pseudomonas aeruginosa |
pitax | - |
Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Pseudomonas aeruginosa |