KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
D-ribose 5-phosphate | pH 7.6, 25°C, affinity to the first active center when the second center is unoccupied, presence of Ca2+ | Saccharomyces cerevisiae | |
0.021 | - |
D-xylulose 5-phosphate | pH 7.6, 25°C, affinity to the first active center when the second center is unoccupied, presence of Ca2+ | Saccharomyces cerevisiae | |
0.115 | - |
D-xylulose 5-phosphate | pH 7.6, 25°C, affinity to the first active center when the second center is unoccupied, presence of Mg2+ | Saccharomyces cerevisiae | |
0.115 | - |
D-xylulose 5-phosphate | pH 7.6, 25°C, affinity to the second active center when the first center is occupied, presence of Mg2+ | Saccharomyces cerevisiae | |
0.4 | - |
D-ribose 5-phosphate | pH 7.6, 25°C, affinity to the first active center when the second center is unoccupied, presence of Mg2+ | Saccharomyces cerevisiae | |
0.4 | - |
D-ribose 5-phosphate | pH 7.6, 25°C, affinity to the second active center when the first center is occupied, presence of Mg2+ | Saccharomyces cerevisiae | |
0.5 | - |
D-xylulose 5-phosphate | pH 7.6, 25°C, affinity to the second active center when the first center is occupied, presence of Ca2+ | Saccharomyces cerevisiae | |
0.6 | - |
D-ribose 5-phosphate | pH 7.6, 25°C, affinity to the second active center when the first center is occupied, presence of Ca2+ | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | in the presence of Ca2+, the enzyme exhibits strong negative active site cooperativity in both xylulose 5-phosphate binding and ribose 5-phosphate binding and positive cooperativity in the catalytic activity | Saccharomyces cerevisiae | |
Mg2+ | nonequivalence of the active sites is not observed in presence of Mg2+ | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylulose 5-phosphate + D-ribose 5-phosphate | in presence of Ca2+, the active centers of the enzyme are nonequivalent with respect to both ribose 5-phosphate and xylulose 5-phosphate binding | Saccharomyces cerevisiae | sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate | - |
? |