Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tRNAfMet | non-aminoacylated tRNAfMet is a competitive inhibitor | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
L-methionyl-tRNAfMet | - |
Escherichia coli | |
0.0013 | - |
phenylalanyl-tRNAfMet | - |
Escherichia coli | |
0.0014 | - |
valyl-tRNAfMet | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Escherichia coli | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | Escherichia coli | involved in initiation of protein biosynthesis | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | Escherichia coli K12 MO | involved in initiation of protein biosynthesis | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
lot 15-290 | - |
Escherichia coli K12 MO | - |
lot 15-290 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + L-methionyl-tRNAfMet | - |
Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
r | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | formylates methionyl-tRNAfMet from E. coli | Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | specific for methionyl-tRNAfMet, specificity depends exclusively on the nature of tRNA moiety of aminoacylated tRNAfMet | Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | involved in initiation of protein biosynthesis | Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | formylates methionyl-tRNAfMet from E. coli | Escherichia coli K12 MO | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | specific for methionyl-tRNAfMet, specificity depends exclusively on the nature of tRNA moiety of aminoacylated tRNAfMet | Escherichia coli K12 MO | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | involved in initiation of protein biosynthesis | Escherichia coli K12 MO | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + phenylalanyl-tRNAfMet + H2O | mischarged E. coli tRNAfMet, reduced velocity compared to the formylation of methionyl-tRNAfMet | Escherichia coli | tetrahydrofolate + N-formylphenylalanyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + phenylalanyl-tRNAfMet + H2O | mischarged E. coli tRNAfMet, reduced velocity compared to the formylation of methionyl-tRNAfMet | Escherichia coli K12 MO | tetrahydrofolate + N-formylphenylalanyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O | mischarged E. coli tRNAfMet | Escherichia coli | tetrahydrofolate + N-formylvalyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O | reduced velocity compared to the formylation of methionyl-tRNAfMet | Escherichia coli | tetrahydrofolate + N-formylvalyl-tRNAfMet | - |
? | |
10-formyltetrahydrofolate + valyl-tRNAfMet + H2O | mischarged E. coli tRNAfMet | Escherichia coli K12 MO | tetrahydrofolate + N-formylvalyl-tRNAfMet | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
10-formyltetrahydrofolate | - |
Escherichia coli | |
tetrahydrofolate | - |
Escherichia coli |