General Stability | Organism |
---|---|
ammonium sulfate fractionation inactivates, resistant to urea denaturation | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | low Km-values for its substrates | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | Salmonella enterica subsp. enterica serovar Typhimurium | first committed step in pantothenate biosynthesis | tetrahydrofolate + 2-dehydropantoate | - |
? | |
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | Escherichia coli | first committed step in pantothenate biosynthesis | tetrahydrofolate + 2-dehydropantoate | enzyme is responsible for catalysis of ketopantoate formation in vivo | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | - |
strains W and B | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
strain K12, 2450fold purification | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.3 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | tetrahydrofolate + 2-dehydropantoate | - |
r | |
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | - |
Escherichia coli | tetrahydrofolate + 2-dehydropantoate | formation of ketopantoate, syn. 2-keto-3,3-dimethyl-4-hydroxybutyrate, tetrahydrofolate-dependent enzyme | r | |
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | first committed step in pantothenate biosynthesis | Salmonella enterica subsp. enterica serovar Typhimurium | tetrahydrofolate + 2-dehydropantoate | - |
? | |
5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | first committed step in pantothenate biosynthesis | Escherichia coli | tetrahydrofolate + 2-dehydropantoate | enzyme is responsible for catalysis of ketopantoate formation in vivo | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
37 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
treatment at 55°C largely destroys enzyme in crude extract, partially purified enzyme is more heat-stabile | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
at or below | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
10 | - |
no ketopantoate formation above pH 10 | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,10-methylenetetrahydrofolate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
5,10-methylenetetrahydrofolate | - |
Escherichia coli | |
tetrahydrofolate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
tetrahydrofolate | tetrahydrofolate-dependent reaction | Escherichia coli |