Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Helicobacter pylori |
Crystallization (Comment) | Organism |
---|---|
apoenzyme, hanging drop vapor diffusion method, using 1.0 M Bis-Tris, pH 9.0, 1.4 M ammonium tartrate. Enzyme in complex with S-adenosyl-L-methionine, hanging drop vapor diffusion method, using 1.0 M Bis-Tris pH 6.0, 14% (w/v) PEG 2000, 0.2 M lithium sulfate | Helicobacter pylori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA adenine | Helicobacter pylori | the enzyme recognizes the sequences of 5'-GAGG-3', 5'-GGAG-3' or 5'-GAAG-3' | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | Helicobacter pylori 26695 | the enzyme recognizes the sequences of 5'-GAGG-3', 5'-GGAG-3' or 5'-GAAG-3' | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
- |
- |
Helicobacter pylori 26695 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity column chromatography, Mono S column chromatography, and Superdex 200 gel filtration | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA adenine | the enzyme recognizes the sequences of 5'-GAGG-3', 5'-GGAG-3' or 5'-GAAG-3' | Helicobacter pylori | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? | |
S-adenosyl-L-methionine + DNA adenine | the enzyme recognizes the sequences of 5'-GAGG-3', 5'-GGAG-3' or 5'-GAAG-3' | Helicobacter pylori 26695 | S-adenosyl-L-homocysteine + DNA 6-methyladenine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | gel filtration | Helicobacter pylori |
Synonyms | Comment | Organism |
---|---|---|
DNA N6-adenine methyltransferase | - |
Helicobacter pylori |
M1.HpyAVI | - |
Helicobacter pylori |
N6-adenine MTase | - |
Helicobacter pylori |