Crystallization (Comment) | Organism |
---|---|
the enzyme in folate-free state, in complex with trimetrexate, and in complex with methotrexate is crystallized by the hanging drop vapor diffusion method, using 16-20% (v/v) 5-methylpentane-2,3-diol, 8-12% (w/v) PEG 4000, 0.1 M potassium citrate pH 6.0, or 1.5-1.7 M ammonium sulfate, 0.01 M magnesium chloride, 5% (w/v) ethylene glycol, 0.05 M Tris pH 7.5, or 17% (v/v) 5-methylpentane-2,3-diol, 10% (w/v) PEG 4000, 0.1 M potassium citrate pH 6.0 | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | Q27793 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | - |
Trypanosoma cruzi | dihydrofolate + dTMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
DHFR-TS | - |
Trypanosoma cruzi |
dihydrofolate reductase-thymidylate synthase | - |
Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
N5,N10-methylenetetrahydrofolate | - |
Trypanosoma cruzi |