Crystallization (Comment) | Organism |
---|---|
- |
Lacticaseibacillus casei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
2'-deoxyuridylate | wild-type | Lacticaseibacillus casei | |
0.0057 | - |
5-bromo-2'-deoxyuridylate | - |
Lacticaseibacillus casei | |
0.0057 | - |
5-bromo-2'-deoxyuridylate | wild-type | Escherichia coli | |
0.0063 | - |
2'-deoxyuridylate | - |
Lacticaseibacillus casei | |
0.0063 | - |
2'-deoxyuridylate | mutant D221S | Escherichia coli | |
0.011 | - |
2'-deoxyuridylate | - |
Lacticaseibacillus casei | |
0.011 | - |
2'-deoxyuridylate | mutant D221E | Escherichia coli | |
0.015 | - |
2'-deoxyuridylate | - |
Lacticaseibacillus casei | |
0.015 | - |
2'-deoxyuridylate | mutant D221C | Escherichia coli | |
0.02 | - |
5-bromo-2'-deoxyuridylate | - |
Lacticaseibacillus casei | |
0.02 | - |
5-bromo-2'-deoxyuridylate | mutant D221A | Escherichia coli | |
0.025 | - |
5-bromo-2'-deoxyuridylate | - |
Lacticaseibacillus casei | |
0.025 | - |
5-bromo-2'-deoxyuridylate | mutant D221C | Escherichia coli | |
0.027 | - |
5-bromo-2'-deoxyuridylate | - |
Lacticaseibacillus casei | |
0.027 | - |
5-bromo-2'-deoxyuridylate | mutant D221S | Escherichia coli | |
0.045 | - |
5-bromo-2'-deoxyuridylate | - |
Lacticaseibacillus casei | |
0.045 | - |
5-bromo-2'-deoxyuridylate | mutant D221N | Escherichia coli | |
0.046 | - |
5-bromo-2'-deoxyuridylate | - |
Lacticaseibacillus casei | |
0.046 | - |
5-bromo-2'-deoxyuridylate | mutant D221E | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | - |
dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Lacticaseibacillus casei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mutant enzymes D221A, N, R, F, C, E, and S | Lacticaseibacillus casei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + 5'-fluoro-2'-deoxyuridine 5'-phosphate | some mutants are capable of forming covalent thymidylate synthase-5-fluoro-dUMP-methylenetetrahydrofolate complex | Lacticaseibacillus casei | 7,8-dihydrofolate + ? | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
5-bromo-2'-deoxyuridine 5'-monophosphate + ? | dehalogenation | Escherichia coli | ? | - |
? | |
5-bromo-2'-deoxyuridine 5'-monophosphate + ? | dehalogenation | Lacticaseibacillus casei | ? | - |
? |