Cloned (Comment) | Organism |
---|---|
gene mtgB or MV8460, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Methanolobus vulcani |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Methanolobus vulcani |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] | Methanolobus vulcani | - |
a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine | - |
? | |
trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] | Methanolobus vulcani B1 | - |
a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine | - |
? | |
trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] | Methanolobus vulcani B1d | - |
a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanolobus vulcani | - |
- |
- |
Methanolobus vulcani B1d | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | proteomics analysis | Methanolobus vulcani | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme MV8460 shows GB:cob(I)alamin methyl-transfer activity. Molecular docking | Methanolobus vulcani | ? | - |
- |
|
additional information | enzyme MV8460 shows GB:cob(I)alamin methyl-transfer activity. Molecular docking | Methanolobus vulcani B1 | ? | - |
- |
|
additional information | enzyme MV8460 shows GB:cob(I)alamin methyl-transfer activity. Molecular docking | Methanolobus vulcani B1d | ? | - |
- |
|
trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] | - |
Methanolobus vulcani | a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine | - |
? | |
trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] | - |
Methanolobus vulcani B1 | a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine | - |
? | |
trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] | - |
Methanolobus vulcani B1d | a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MtgB | - |
Methanolobus vulcani |
MV8460 | - |
Methanolobus vulcani |
MV8460 GB:cob(I)alamin | - |
Methanolobus vulcani |
non-pyrrolysine MttB homolog | - |
Methanolobus vulcani |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanolobus vulcani |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Methanolobus vulcani |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the COG5598 MttB superfamily. Based on the draft B1d genome, MV8460 is the sole Pyl-lacking MttB found in B1d, which is 65% identical and has 83% sequence similarity to Desulfitobacterium hafniense Y51 DhMtgB. Four predicted active site residues may interact with GB as they are conserved between DhMtgB and MV8460: Y94, N199, R309, and H345 | Methanolobus vulcani |
metabolism | the enzyme is involved in the the glycine betaine-dependent methylotrophic methanogenesis pathway. Environmentally abundant quaternary amines (QAs) are a primary source for methanogenesis. The methanogenic archaeon Methanolobus vulcani B1d metabolizes glycine betaine (GB) through a corrinoid-dependent GB:coenzyme M (CoM) methyl transfer pathway | Methanolobus vulcani |
physiological function | MV8460 catalyzes GB-dependent methylation of free cob(I) alamin indicating it is an authentic MtgB enzyme | Methanolobus vulcani |