Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus abyssi |
Crystallization (Comment) | Organism |
---|---|
concentrated by ultrafiltration, crystallisation at 20°C by the sitting-drop vapour diffusion method | Pyrococcus abyssi |
Protein Variants | Comment | Organism |
---|---|---|
C196S/C233S | mutant enzyme C196S/C233S is inactivated at 85°C after 20 min, wild-type enzyme is stable for more than 1 h | Pyrococcus abyssi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
4 * 30000, SDS-PAGE under reducing conditions | Pyrococcus abyssi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + adenine57/adenine58 in tRNA | Pyrococcus abyssi | - |
2 S-adenosyl-L-homocysteine + N1-methyladenine57/N1-methyladenine58 in tRNA | - |
? | |
2 S-adenosyl-L-methionine + adenine57/adenine58 in tRNA | Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | - |
2 S-adenosyl-L-homocysteine + N1-methyladenine57/N1-methyladenine58 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus abyssi | Q9V1J7 | - |
- |
Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | Q9V1J7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus abyssi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + adenine57/adenine58 in tRNA | - |
Pyrococcus abyssi | 2 S-adenosyl-L-homocysteine + N1-methyladenine57/N1-methyladenine58 in tRNA | - |
? | |
2 S-adenosyl-L-methionine + adenine57/adenine58 in tRNA | - |
Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | 2 S-adenosyl-L-homocysteine + N1-methyladenine57/N1-methyladenine58 in tRNA | - |
? | |
2 S-adenosyl-L-methionine + adenine57/adenine58 in tRNAAsp | - |
Pyrococcus abyssi | 2 S-adenosyl-L-homocysteine + N1-methyladenine57/N1-methyladenine58 in tRNAAsp | - |
? | |
2 S-adenosyl-L-methionine + adenine57/adenine58 in tRNAAsp | - |
Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | 2 S-adenosyl-L-homocysteine + N1-methyladenine57/N1-methyladenine58 in tRNAAsp | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 30000, SDS-PAGE under reducing conditions | Pyrococcus abyssi |
Synonyms | Comment | Organism |
---|---|---|
TrmI | - |
Pyrococcus abyssi |
tRNA (m1A) methyltransferase | - |
Pyrococcus abyssi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the stabilisation of Pyrococcus abyssi TrmI at extreme temperatures involves intersubunit disulfide bridges formed between Cys196 and Cys233 that reinforce the tetrameric oligomerisation | Pyrococcus abyssi |
80 | - |
1 h, wild-type enzyme and mutant enzyme C196S/C233S are stable | Pyrococcus abyssi |
85 | - |
mutant enzyme C196S/C233S is inactivated after 20 min, wild-type enzyme is stable for more than 1 h | Pyrococcus abyssi |