Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli and the His-tagged Trm1 protein | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
E47A | mutant enzyme shows 6% of wild-type activity | Saccharomyces cerevisiae |
G48A | mutant enzyme shows 16% of wild-type activity | Saccharomyces cerevisiae |
additional information | comparatively short N- or C-terminal deletions from the 570 amino acid long Trm1 polypeptide decreased or eliminated the enzyme activity. The first 27 N-terminal amino acids in rTrmp1 are not needed for G26 modification activity in vitro or in vivo. DELTAC5aa causes the in vitro modification activity to drastically decrease to 27% of the wild-type rate. Additional deletions up to DELTA30aa further lower the activity to about 7% of the wild-type value. In DELTA10aa an increase of the N2-methylguanine26 relative to N2-dimethylguanine26 tRNA is observed, but with further C-terminal deletions the monomethylation activity is also severely lowered | Saccharomyces cerevisiae |
S467L | complete loss of enzyme activity in vitro against trm1 yeast tRNA | Saccharomyces cerevisiae |
S570A | fully active mutant enzyme | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64050 | - |
calculated from sequence | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P15565 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
4°C, the enzyme precipitates if stored for several days | Saccharomyces cerevisiae |
frozen in small portions, enzyme maintains activity for at least several months | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + guanine26 in tRNA | wild-type enzyme produces 94% N2-dimethylguanine26 tRNA and 6% N2-methylguanine26 in tRNA | Saccharomyces cerevisiae | 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA | - |
? | |
S-adenosyl-L-methionine + guanine26 in tRNA | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA | - |
? | |
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Trm1 | - |
Saccharomyces cerevisiae |
tRNA (m22G26)dimethyltransferase | - |
Saccharomyces cerevisiae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharomyces cerevisiae | calculated from sequence | - |
9.2 |