Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapour diffusion method in reservoir solution of 0.1 M Tris/HCl pH 7.5 containing 0.1-0.4 M sodium acetate, 10-12% poly(ethylene glycol) 8000 and 10-12% poly(ethyleneglycol) 1000 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D963E /K1071N | reduced activity | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36200 | - |
sedimentation in high salt buffer | Homo sapiens |
40000 | - |
2 * 40000, SDS-PAGE | Homo sapiens |
43100 | - |
1 * 43100, light scattering | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin HisTrap column chromatography, HiPrep Q Sepharose column chromatography, and Resource Q column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N5-methyltetrahydropteroylmonoglutamate + L-homocysteine | - |
Homo sapiens | tetrahydropteroylmonoglutamate + L-methionine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE | Homo sapiens |
monomer | 1 * 43100, light scattering | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
5-methyltetrahydrofolate homocysteine methyltransferase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
vitamin B12 | dependent | Homo sapiens |