Literature summary for 2.1.1.111 extracted from

Lee, H.L.; Kim, S.Y.; Kim, E.J.; Han, D.Y.; Kim, B.G.; Ahn, J.H.
Synthesis of methylated anthranilate derivatives using engineered strains of Escherichia coli (2019), J. Microbiol. Biotechnol., 29, 839-844 .

Search for more literature for 2.1.1.111

Application

Application	Comment	Organism
synthesis	expression of anthranilate N-methyltransferase, anthraniloyl-coenzyme A (CoA):methanol acyltransferase from Vitis labrusca, and anthranilate coenzyme A ligase from Pseudomonas aeruginosa in Escherichia coli mutants mutants (metJ, trpD, tyrR), which provide more anthranilate and/or S-adenosyl methionine, to synthesize N-methylanthranilate, methyl anthranilate, and methyl N-methylanthranilate. Approximately, 185.3 microM N-methylanthranilate and 95.2 microM methyl N-methylanthranilate are synthesized	Ruta graveolens
synthesis	three anthranilate derivatives, N-methylanthranilate, methyl anthranilate, and methyl N-methylanthranilate are synthesized using metabolically engineered stains of Escherichia coli. NMT encoding N-methyltransferase from Ruta graveolens, AMAT encoding anthraniloyl-coenzyme A (CoA):methanol acyltransferase from Vitis labrusca, and pqsA encoding anthranilate coenzyme A ligase from Pseudomonas aeruginosa are cloned and Escherichia coli strains harboring these genes were used to synthesize the three desired compounds. Escherichia coli mutants (metJ, trpD, tyrR mutants), which provide more anthranilate and/or S-adenosyl methionine, are used to increase the production of the synthesized compounds. 0.1853 mM N-methylanthranilate and 0.0952 mM methyl N-methylanthranilate are synthesized	Ruta graveolens

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Ruta graveolens

Organism

Organism	UniProt	Comment	Textmining
Ruta graveolens	A9X7L0	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + anthranilate	-	Ruta graveolens	S-adenosyl-L-homocysteine + N-methylanthranilate	-	?
S-adenosyl-L-methionine + methyl anthranilate	-	Ruta graveolens	S-adenosyl-L-homocysteine + methyl N-methylanthranilate	-	?

Synonyms

Synonyms	Comment	Organism
NMT	-	Ruta graveolens

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Release 2023.1 (January 2023)