Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C103A | inactive | Escherichia coli |
C109A | inactive | Escherichia coli |
C163A | inactive | Escherichia coli |
C282A | inactive | Escherichia coli |
C464A | inactive | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasmic membrane | - |
Escherichia coli | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a [protein] carrying a disulfide bond + thioredoxin | Escherichia coli | overall reaction | a [protein] with reduced L-cysteine residues + thioredoxin disulfide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Mono-Q Sepharose column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a [protein] carrying a disulfide bond + thioredoxin | overall reaction | Escherichia coli | a [protein] with reduced L-cysteine residues + thioredoxin disulfide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
DsbD | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | cytoplasmic electrons donated by thioredoxin are transferred into the periplasm via the enzyme | Escherichia coli |