Cloned (Comment) | Organism |
---|---|
msrA, DNA and amino acid sequence determination and analysis, expression of C-terminally His-tagged or GFP-tagged wild-type or truncated MsrA in Escherichia coli strain BL21(DE3), expression of MsrA isozymes in Saccharomyces cerevisiae, subcellular localization of the recombinant enzymes in cytosol and mitochondria, overview | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of the truncated MsrA DELTA(1-46) mutant | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
dabsyl-L-methionine-(S)-S-oxide | recombinant wild-type MsrA, with DTT, pH 7.5, 37°C | Mus musculus | |
4.3 | - |
dabsyl-L-methionine-(S)-S-oxide | recombinant truncated MsrA DELTA(1-46), with DTT, pH 7.5, 37°C | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
mitochondrion | - |
Mus musculus | 5739 | - |
additional information | post-translationally occuring subcellular targeting and distribution of isozymes, overview, several forms are generated from a single translation form | Mus musculus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptide-L-methionine-(S)-S-oxide + thioredoxin | Mus musculus | MsrA is involved in repair of oxidized proteins | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.099 | - |
purified recombinant truncated MsrA DELTA(1-46), substrates DTT and dabsyl-L-methionine-(S)-S-oxide | Mus musculus |
0.238 | - |
purified recombinant wild-type MsrA, substrates DTT and dabsyl-L-methionine-(S)-S-oxide | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dabsyl-L-methionine-(S)-S-oxide + DTT | stereospecific reduction | Mus musculus | dabsyl-L-methionine + DTT disulfide + H2O | - |
? | |
additional information | role of subcellular localization in structure-function relationship of the isozymes, overview | Mus musculus | ? | - |
? | |
peptide-L-methionine-(S)-S-oxide + thioredoxin | stereospecific reduction | Mus musculus | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? | |
peptide-L-methionine-(S)-S-oxide + thioredoxin | MsrA is involved in repair of oxidized proteins | Mus musculus | peptide-L-methionine + thioredoxin disulfide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methionine-S-sulfoxide reductase | - |
Mus musculus |
MsrA | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
dabsyl-L-methionine-(S)-S-oxide | recombinant wild-type MsrA, with DTT, pH 7.5, 37°C | Mus musculus | |
0.78 | - |
dabsyl-L-methionine-(S)-S-oxide | recombinant truncated MsrA DELTA(1-46), with DTT, pH 7.5, 37°C | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Mus musculus | |
thioredoxin | - |
Mus musculus |