Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | inhibition of dialyzed enzyme at 0.0005 mM | Pseudomonas aeruginosa | |
hydrazine | competitive inhibition | Pseudomonas aeruginosa | |
Zn2+ | inhibition of dialyzed enzyme at 0.0005 mM | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000004 | - |
hydroxylamine | - |
Pseudomonas aeruginosa | |
0.00001 | - |
FADH2 | - |
Pseudomonas aeruginosa | |
0.000012 | - |
reduced pyocyanine | - |
Pseudomonas aeruginosa | |
0.000044 | - |
reduced methylene blue | - |
Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can substitute for Mn2+, only 10% as effective | Pseudomonas aeruginosa | |
Mn2+ | required for maximal activity | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.87 | - |
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxylamine + reduced acceptor | with pyocyanine or reduced methylene blue as electron donor | Pseudomonas aeruginosa | ammonia + oxidized acceptor | - |
? | |
nitrate + FADH2 | - |
Pseudomonas aeruginosa | nitrite + FAD + H2O | - |
r | |
nitrate + reduced methylene blue | - |
Pseudomonas aeruginosa | nitrite + H2O + oxidized methylene blue | - |
r | |
nitrate + reduced pyocyanine | - |
Pseudomonas aeruginosa | nitrite + H2O + oxidized pyocyanine | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein, contains 0.9 nmol FAD per mg protein | Pseudomonas aeruginosa |