Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Synechococcus elongatus PCC 7942 = FACHB-805 |
Protein Variants | Comment | Organism |
---|---|---|
E219Q | the mutant shows 96.1% activity when reduced ferredoxin serves as the electron donor compared to the wild type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |
K201Q | the mutant shows 14.8% activity and decreased binding affinity when reduced ferredoxin serves as the electron donor compared to the wild type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |
K614Q | the mutant shows 11.5% activity and decreased binding affinity when reduced ferredoxin serves as the electron donor compared to the wild type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |
K614R | the mutant shows 63.3% activity when reduced ferredoxin serves as the electron donor compared to the wild type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |
R197Q | the mutant shows 91.8% activity when reduced ferredoxin serves as the electron donor compared to the wild type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |
R43K | the mutant shows 91% activity when reduced ferredoxin serves as the electron donor compared to the wild type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |
R43Q | the mutant shows 16.4% activity and decreased binding affinity when reduced ferredoxin serves as the electron donor compared to the wild type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |
R46K | the mutant shows 97.7% activity when reduced ferredoxin serves as the electron donor compared to the wild type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |
R46Q | the mutant 24.3% activity and decreased binding affinity when reduced ferredoxin serves as the electron donor compared to the wild type enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0042 | - |
nitrate | mutant enzyme K614R, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.0045 | - |
nitrate | mutant enzyme E219QR, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.006 | - |
nitrate | wild type enzyme, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.0066 | - |
nitrate | mutant enzyme R46K, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.007 | - |
nitrate | mutant enzyme R197Q, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.0081 | - |
nitrate | mutant enzyme R43K, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.0096 | - |
nitrate | mutant enzyme K201R, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.038 | - |
reduced ferredoxin | wild type enzyme, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.0419 | - |
reduced ferredoxin | mutant enzyme R197Q, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.0422 | - |
reduced ferredoxin | mutant enzyme E219QR, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.0445 | - |
reduced ferredoxin | mutant enzyme K614R, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.0456 | - |
reduced ferredoxin | mutant enzyme K201R, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.0486 | - |
reduced ferredoxin | mutant enzyme R43K, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.0496 | - |
reduced ferredoxin | mutant enzyme R46K, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.055 | - |
nitrate | mutant enzyme R46Q, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 | |
0.364 | - |
reduced ferredoxin | mutant enzyme R46Q, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the enzyme contains 3.6 moles iron per mole of enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 | |
Molybdenum | the enzyme contains 0.93 moles molybdenum per mole of enzyme | Synechococcus elongatus PCC 7942 = FACHB-805 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + 2 reduced ferredoxin + 2 H+ | Synechococcus elongatus PCC 7942 = FACHB-805 | - |
nitrite + H2O + 2 oxidized ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechococcus elongatus PCC 7942 = FACHB-805 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.5 | - |
mutant enzyme K614Q, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 |
4.5 | - |
mutant enzyme K201Q, with reduced ferredoxin as electron donor, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 |
5 | - |
mutant enzyme R43Q, with reduced ferredoxin as electron donor, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 |
7.4 | - |
mutant enzyme R46Q, with reduced ferredoxin as electron donor, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 |
19.3 | - |
mutant enzyme K614R, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 |
27.8 | - |
mutant enzyme R43K, with reduced ferredoxin as electron donor, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 |
27.9 | - |
mutant enzyme R197Q, with reduced ferredoxin as electron donor, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 |
29.3 | - |
mutant enzyme E219Q, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 |
29.4 | - |
mutant enzyme K201R, with reduced ferredoxin as electron donor, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 |
29.8 | - |
mutant enzyme R46K, with reduced ferredoxin as electron donor, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 |
30.5 | - |
wild type enzyme, with reduced ferredoxin as electron donor, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 |
721 | - |
mutant enzyme R43Q, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 |
734 | - |
mutant enzyme R43K, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 |
738 | - |
mutant enzyme R197Q, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 |
749 | - |
mutant enzyme E219Q, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 |
761 | - |
mutant enzyme K614R, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 |
768 | - |
mutant enzyme R46Q, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 |
771 | - |
mutant enzyme K614Q, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 |
775 | - |
mutant enzyme K201R, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 |
779 | - |
mutant enzyme K201Q, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 |
783 | - |
mutant enzyme R46K, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 |
790 | - |
wild type enzyme, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication | Synechococcus elongatus PCC 7942 = FACHB-805 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + 2 reduced ferredoxin + 2 H+ | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | nitrite + H2O + 2 oxidized ferredoxin | - |
? | |
nitrate + 2 reduced methyl viologen + 2 H+ | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | nitrite + H2O + 2 oxidized methyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 78000, SDS-PAGE | Synechococcus elongatus PCC 7942 = FACHB-805 |
Synonyms | Comment | Organism |
---|---|---|
Fd-dependent nitrate reductase | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |
narB | - |
Synechococcus elongatus PCC 7942 = FACHB-805 |