Activating Compound | Comment | Organism | Structure |
---|---|---|---|
hydroxylamine | 1 mM, 108% of initial activity | Pseudomonas oleovorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-Naphthylamine | 1 mM, 31% residual activity | Pseudomonas oleovorans | |
CuSO4 | 0.5 mM, 78% residual activity | Pseudomonas oleovorans | |
cysteine | 1 mM, 87% residual activity | Pseudomonas oleovorans | |
dicumarol | 0.01 mM, 40% residual activity | Pseudomonas oleovorans | |
N-ethylmaleimide | 1 mM, 77% residual activity | Pseudomonas oleovorans | |
neocuproin | 1 mM, 39% residual activity | Pseudomonas oleovorans | |
nitrite | 1 mM, 77% residual activity | Pseudomonas oleovorans | |
p-hydroxymercuribenzoate | 1 mM, 46% residual activity | Pseudomonas oleovorans | |
potassium cyanate | 0.5 mM, 75% residual activity | Pseudomonas oleovorans | |
salicyl hydroxamate | 1 mM, 6% residual activity | Pseudomonas oleovorans | |
Sodium azide | 1 mM, 35% residual activity | Pseudomonas oleovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
nitrobenzene | temperature not specified in the publication, pH 8.0 | Pseudomonas oleovorans | |
0.009 | - |
menadione | temperature not specified in the publication, pH 8.0 | Pseudomonas oleovorans | |
0.183 | - |
NADPH | temperature not specified in the publication, pH 8.0 | Pseudomonas oleovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel fitration | Pseudomonas oleovorans |
33000 | - |
1 * 33000, SDS-PAGE | Pseudomonas oleovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrobenzene + 2 NADPH + 2 H+ | Pseudomonas oleovorans | - |
N-hydroxyaniline + 2 NADP+ + H2O | N-hydroxyaniline is the only product detected | ? | |
nitrobenzene + 2 NADPH + 2 H+ | Pseudomonas oleovorans JS45 | - |
N-hydroxyaniline + 2 NADP+ + H2O | N-hydroxyaniline is the only product detected | ? | |
nitrosobenzene + NADPH + H+ | Pseudomonas oleovorans | - |
N-hydroxyaniline + NADP+ + H2O | nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme | ? | |
nitrosobenzene + NADPH + H+ | Pseudomonas oleovorans JS45 | - |
N-hydroxyaniline + NADP+ + H2O | nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas oleovorans | Q6DLR9 | - |
- |
Pseudomonas oleovorans JS45 | Q6DLR9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas oleovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
245 | - |
temperature not specified in the publication, pH 8.0 | Pseudomonas oleovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | 1.8 mol of NADPH is oxidized per mol of nitrobenzene converted to product | Pseudomonas oleovorans | ? | - |
? | |
additional information | 1.8 mol of NADPH is oxidized per mol of nitrobenzene converted to product | Pseudomonas oleovorans JS45 | ? | - |
? | |
nitrobenzene + 2 NADPH + 2 H+ | - |
Pseudomonas oleovorans | N-hydroxyaniline + 2 NADP+ + H2O | N-hydroxyaniline is the only product detected | ? | |
nitrobenzene + 2 NADPH + 2 H+ | - |
Pseudomonas oleovorans JS45 | N-hydroxyaniline + 2 NADP+ + H2O | N-hydroxyaniline is the only product detected | ? | |
nitrobenzene + menadione | - |
Pseudomonas oleovorans | N-hydroxyaniline + menadiol + H2O | - |
? | |
nitrobenzene + menadione | - |
Pseudomonas oleovorans JS45 | N-hydroxyaniline + menadiol + H2O | - |
? | |
nitrosobenzene + NADPH + H+ | - |
Pseudomonas oleovorans | N-hydroxyaniline + NADP+ + H2O | nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme | ? | |
nitrosobenzene + NADPH + H+ | - |
Pseudomonas oleovorans JS45 | N-hydroxyaniline + NADP+ + H2O | nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 33000, SDS-PAGE | Pseudomonas oleovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | tightly bound flavin mononucleotide cofactor in a ratio of 2 mol of flavin per mol of protein | Pseudomonas oleovorans | |
additional information | no cofactor: NADH | Pseudomonas oleovorans | |
NADPH | - |
Pseudomonas oleovorans |