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Literature summary for 1.5.3.17 extracted from

  • Kim, D.W.; Watanabe, K.; Murayama, C.; Izawa, S.; Niitsu, M.; Michael, A.J.; Berberich, T.; Kusano, T.
    Polyamine oxidase 5 regulates Arabidopsis growth through Thermospermine oxidase activity (2014), Plant Physiol., 165, 1575-1590 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0019
-
N1-acetylspermine 37°C, pH 7.5 Arabidopsis thaliana
0.0051
-
thermospermine 37°C, pH 7.5 Arabidopsis thaliana
0.0137
-
thermospermine 37°C, pH 6.5 Arabidopsis thaliana
0.0158
-
norspermine 37°C, pH 6.5 Arabidopsis thaliana
0.0218
-
N1-acetylspermine 37°C, pH 6.5 Arabidopsis thaliana
0.0255
-
norspermine 37°C, pH 7.5 Arabidopsis thaliana
0.0256
-
spermine 37°C, pH 7.5 Arabidopsis thaliana
0.0683
-
spermidine 37°C, pH 7.5 Arabidopsis thaliana
0.0787
-
spermine 37°C, pH 6.5 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thermospermine + O2 + H2O Arabidopsis thaliana
-
?
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9SU79
-
-

Source Tissue

Source Tissue Comment Organism Textmining
procambium
-
Arabidopsis thaliana
-
vascular bundle
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N1-acetylspermine + O2 + H2O at pH 6.5, substrate preference is in the following decreasing order: thermospermine, N1-acetylspermine, norspermine, spermine, spermidine. Spermidine is catabolized at a very low rate. At pH 7.5, substrate preference is in the following decreasing order: spermine, norspermine, N1-acetylspermine, thermospermine, spermidine. Spermidine is catalyzed at a very low rate Arabidopsis thaliana spermidine + 3-acetamidopropanal + H2O2
-
?
norspermine + O2 + H2O at pH 6.5, substrate preference is in the following decreasing order: thermospermine, N1-acetylspermine, norspermine, spermine, spermidine. Spermidine is catabolized at a very low rate. At pH 7.5, substrate preference is in the following decreasing order: spermine, norspermine, N1-acetylspermine, thermospermine, spermidine. Spermidine is catalyzed at a very low rate Arabidopsis thaliana ?
-
?
spermidine + O2 + H2O at pH 6.5, substrate preference is in the following decreasing order: thermospermine, N1-acetylspermine, norspermine, spermine, spermidine. Spermidine is catabolized at a very low rate. At pH 7.5, substrate preference is in the following decreasing order: spermine, norspermine, N1-acetylspermine, thermospermine, spermidine. Spermidine is catalyzed at a very low rate Arabidopsis thaliana putrescine + 3-aminopropanal + H2O2
-
?
spermine + O2 + H2O at pH 6.5, substrate preference is in the following decreasing order: thermospermine, N1-acetylspermine, norspermine, spermine, spermidine. Spermidine is catabolized at a very low rate. At pH 7.5, substrate preference is in the following decreasing order: spermine, norspermine, N1-acetylspermine, thermospermine, spermidine. Spermidine is catalyzed at a very low rate Arabidopsis thaliana spermidine + 3-aminopropanal + H2O2
-
?
thermospermine + O2 + H2O
-
Arabidopsis thaliana ?
-
?
thermospermine + O2 + H2O at pH 6.5, substrate preference is in the following decreasing order: thermospermine, N1-acetylspermine, norspermine, spermine, spermidine. Spermidine is catabolized at a very low rate. At pH 7.5, substrate preference is in the following decreasing order: spermine, norspermine, N1-acetylspermine, thermospermine, spermidine. Spermidine is catalyzed at a very low rate. At pH 6.5, all thermospermine substrate is converted to spermidine within 30 min. A very low putrescine level is detected at 30 min. The conversion of thermospermine to spermidine is reduced at pH 6.0, and only tiny amounts of putrescine are observed Arabidopsis thaliana spermidine + ?
-
?

Synonyms

Synonyms Comment Organism
AtPAO5
-
Arabidopsis thaliana
T-Spm oxidase
-
Arabidopsis thaliana
thermospermine oxidase
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 42 substrate: spermine Arabidopsis thaliana
45
-
substrate: thermospermine Arabidopsis thaliana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 25°C: about 45% of maximal activity, 50°C: about 90% of maximal activity, substrate: thermospermine Arabidopsis thaliana
25 50 25°C: about 55% of maximal activity, 50°C: about 45% of maximal activity, substrate: spermine Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.009
-
spermidine 37°C, pH 7.5 Arabidopsis thaliana
0.009
-
spermine 37°C, pH 6.5 Arabidopsis thaliana
0.012
-
norspermine 37°C, pH 6.5 Arabidopsis thaliana
0.025
-
N1-acetylspermine 37°C, pH 6.5 Arabidopsis thaliana
0.035
-
thermospermine 37°C, pH 6.5 Arabidopsis thaliana
0.069
-
spermine 37°C, pH 7.5 Arabidopsis thaliana
0.084
-
N1-acetylspermine 37°C, pH 7.5 Arabidopsis thaliana
0.086
-
norspermine 37°C, pH 7.5 Arabidopsis thaliana
0.115
-
thermospermine 37°C, pH 7.5 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
substrate: thermospermine Arabidopsis thaliana
7.5
-
substrate: spermine Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
6 10 pH 6.0: about 65% of maximal activity, pH 10.0: about 35% of maximal activity, substrate: thermospermine Arabidopsis thaliana
7 9 pH 7.0: about 60% of maximal activity, pH 9.0: about 45% of maximal activity, substrate: spermine Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction loss-of-function mutants contain 2-fold higher thermospermine levels and exhibit delayed transition from vegetative to reproductive growth compared with that of wild-type plants Arabidopsis thaliana
physiological function the enzyme regulates thermospermine homeostasis through a thermospermine oxidation pathway Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.115
-
spermine 37°C, pH 6.5 Arabidopsis thaliana
0.185
-
spermidine 37°C, pH 7.5 Arabidopsis thaliana
0.747
-
norspermine 37°C, pH 6.5 Arabidopsis thaliana
2.6
-
thermospermine 37°C, pH 6.5 Arabidopsis thaliana
2.7
-
spermine 37°C, pH 7.5 Arabidopsis thaliana
3.4
-
norspermine 37°C, pH 7.5 Arabidopsis thaliana
12.3
-
N1-acetylspermine 37°C, pH 6.5 Arabidopsis thaliana
23.3
-
thermospermine 37°C, pH 7.5 Arabidopsis thaliana
44
-
N1-acetylspermine 37°C, pH 7.5 Arabidopsis thaliana