KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
N1-acetylspermine | 37°C, pH 7.5 | Arabidopsis thaliana | |
0.0051 | - |
thermospermine | 37°C, pH 7.5 | Arabidopsis thaliana | |
0.0137 | - |
thermospermine | 37°C, pH 6.5 | Arabidopsis thaliana | |
0.0158 | - |
norspermine | 37°C, pH 6.5 | Arabidopsis thaliana | |
0.0218 | - |
N1-acetylspermine | 37°C, pH 6.5 | Arabidopsis thaliana | |
0.0255 | - |
norspermine | 37°C, pH 7.5 | Arabidopsis thaliana | |
0.0256 | - |
spermine | 37°C, pH 7.5 | Arabidopsis thaliana | |
0.0683 | - |
spermidine | 37°C, pH 7.5 | Arabidopsis thaliana | |
0.0787 | - |
spermine | 37°C, pH 6.5 | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thermospermine + O2 + H2O | Arabidopsis thaliana | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SU79 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
procambium | - |
Arabidopsis thaliana | - |
vascular bundle | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N1-acetylspermine + O2 + H2O | at pH 6.5, substrate preference is in the following decreasing order: thermospermine, N1-acetylspermine, norspermine, spermine, spermidine. Spermidine is catabolized at a very low rate. At pH 7.5, substrate preference is in the following decreasing order: spermine, norspermine, N1-acetylspermine, thermospermine, spermidine. Spermidine is catalyzed at a very low rate | Arabidopsis thaliana | spermidine + 3-acetamidopropanal + H2O2 | - |
? | |
norspermine + O2 + H2O | at pH 6.5, substrate preference is in the following decreasing order: thermospermine, N1-acetylspermine, norspermine, spermine, spermidine. Spermidine is catabolized at a very low rate. At pH 7.5, substrate preference is in the following decreasing order: spermine, norspermine, N1-acetylspermine, thermospermine, spermidine. Spermidine is catalyzed at a very low rate | Arabidopsis thaliana | ? | - |
? | |
spermidine + O2 + H2O | at pH 6.5, substrate preference is in the following decreasing order: thermospermine, N1-acetylspermine, norspermine, spermine, spermidine. Spermidine is catabolized at a very low rate. At pH 7.5, substrate preference is in the following decreasing order: spermine, norspermine, N1-acetylspermine, thermospermine, spermidine. Spermidine is catalyzed at a very low rate | Arabidopsis thaliana | putrescine + 3-aminopropanal + H2O2 | - |
? | |
spermine + O2 + H2O | at pH 6.5, substrate preference is in the following decreasing order: thermospermine, N1-acetylspermine, norspermine, spermine, spermidine. Spermidine is catabolized at a very low rate. At pH 7.5, substrate preference is in the following decreasing order: spermine, norspermine, N1-acetylspermine, thermospermine, spermidine. Spermidine is catalyzed at a very low rate | Arabidopsis thaliana | spermidine + 3-aminopropanal + H2O2 | - |
? | |
thermospermine + O2 + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
thermospermine + O2 + H2O | at pH 6.5, substrate preference is in the following decreasing order: thermospermine, N1-acetylspermine, norspermine, spermine, spermidine. Spermidine is catabolized at a very low rate. At pH 7.5, substrate preference is in the following decreasing order: spermine, norspermine, N1-acetylspermine, thermospermine, spermidine. Spermidine is catalyzed at a very low rate. At pH 6.5, all thermospermine substrate is converted to spermidine within 30 min. A very low putrescine level is detected at 30 min. The conversion of thermospermine to spermidine is reduced at pH 6.0, and only tiny amounts of putrescine are observed | Arabidopsis thaliana | spermidine + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AtPAO5 | - |
Arabidopsis thaliana |
T-Spm oxidase | - |
Arabidopsis thaliana |
thermospermine oxidase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 42 | substrate: spermine | Arabidopsis thaliana |
45 | - |
substrate: thermospermine | Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | 25°C: about 45% of maximal activity, 50°C: about 90% of maximal activity, substrate: thermospermine | Arabidopsis thaliana |
25 | 50 | 25°C: about 55% of maximal activity, 50°C: about 45% of maximal activity, substrate: spermine | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
spermidine | 37°C, pH 7.5 | Arabidopsis thaliana | |
0.009 | - |
spermine | 37°C, pH 6.5 | Arabidopsis thaliana | |
0.012 | - |
norspermine | 37°C, pH 6.5 | Arabidopsis thaliana | |
0.025 | - |
N1-acetylspermine | 37°C, pH 6.5 | Arabidopsis thaliana | |
0.035 | - |
thermospermine | 37°C, pH 6.5 | Arabidopsis thaliana | |
0.069 | - |
spermine | 37°C, pH 7.5 | Arabidopsis thaliana | |
0.084 | - |
N1-acetylspermine | 37°C, pH 7.5 | Arabidopsis thaliana | |
0.086 | - |
norspermine | 37°C, pH 7.5 | Arabidopsis thaliana | |
0.115 | - |
thermospermine | 37°C, pH 7.5 | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
substrate: thermospermine | Arabidopsis thaliana |
7.5 | - |
substrate: spermine | Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | pH 6.0: about 65% of maximal activity, pH 10.0: about 35% of maximal activity, substrate: thermospermine | Arabidopsis thaliana |
7 | 9 | pH 7.0: about 60% of maximal activity, pH 9.0: about 45% of maximal activity, substrate: spermine | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | loss-of-function mutants contain 2-fold higher thermospermine levels and exhibit delayed transition from vegetative to reproductive growth compared with that of wild-type plants | Arabidopsis thaliana |
physiological function | the enzyme regulates thermospermine homeostasis through a thermospermine oxidation pathway | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.115 | - |
spermine | 37°C, pH 6.5 | Arabidopsis thaliana | |
0.185 | - |
spermidine | 37°C, pH 7.5 | Arabidopsis thaliana | |
0.747 | - |
norspermine | 37°C, pH 6.5 | Arabidopsis thaliana | |
2.6 | - |
thermospermine | 37°C, pH 6.5 | Arabidopsis thaliana | |
2.7 | - |
spermine | 37°C, pH 7.5 | Arabidopsis thaliana | |
3.4 | - |
norspermine | 37°C, pH 7.5 | Arabidopsis thaliana | |
12.3 | - |
N1-acetylspermine | 37°C, pH 6.5 | Arabidopsis thaliana | |
23.3 | - |
thermospermine | 37°C, pH 7.5 | Arabidopsis thaliana | |
44 | - |
N1-acetylspermine | 37°C, pH 7.5 | Arabidopsis thaliana |