Cloned (Comment) | Organism |
---|---|
AtPAO3, expression as GFP-tagged enzyme in plant cells, expression of AtPAO3 fused to the maltose-binding protein in Escherichia coli strain BL21 in a temperature-dependent manner, overview | Arabidopsis thaliana |
expression in Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aminoguanidine | - |
Arabidopsis thaliana | |
guazatine | - |
Arabidopsis thaliana | |
N8-Acetylspermidine | - |
Arabidopsis thaliana | |
putrescine | - |
Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
N1-acetylspermine | pH 7.5, 37°C, recombinant AtPAO3 | Arabidopsis thaliana | |
0.042 | - |
N1-acetylspermidine | pH 7.5, 37°C, recombinant AtPAO3 | Arabidopsis thaliana | |
0.204 | - |
spermidine | 37°C, pH 7.0 | Arabidopsis thaliana | |
0.204 | - |
spermidine | pH 7.5, 37°C, recombinant AtPAO3 | Arabidopsis thaliana | |
0.588 | - |
spermine | 37°C, pH 7.0 | Arabidopsis thaliana | |
0.588 | - |
spermine | pH 7.5, 37°C, recombinant AtPAO3 | Arabidopsis thaliana | |
1 | - |
N1-acetylspermidine | 37°C, pH 7.0 | Arabidopsis thaliana | |
2 | - |
N1-acetylspermine | 37°C, pH 7.0 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | isozyme AtPAO3 contains a peroxisomal targeting motif at the C terminus | Arabidopsis thaliana | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54100 | - |
x * 54100, calculated from sequence | Arabidopsis thaliana |
54100 | - |
x * 54100, AtPAO3, sequence calculation | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | AtPAO3 catalyzes the sequential conversion/oxidation of spermine to spermidine, and of spermidine to putrescine, thus exhibiting functional homology to the mammalian PAOs, but AtPAO3 does not catalyze the conversion of putrescine back to spermine | ? | - |
? | |
spermidine + O2 + H2O | Arabidopsis thaliana | - |
putrescine + 3-aminopropanal + H2O2 | - |
? | |
spermine + O2 + H2O | Arabidopsis thaliana | - |
spermidine + 3-aminopropanal + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9LYT1 | - |
- |
Arabidopsis thaliana | Q9LYT1 | isozyme AtPAO3 | - |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
recombinant AtPAO3 fused to the maltose-binding protein from Escherichia coli | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | mostly expressed in the leaves | Arabidopsis thaliana | - |
leaf | most expression of AtPAO3 | Arabidopsis thaliana | - |
root | low expression of AtPAO3 | Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with agmatine, cadaverine, and putrescine | Arabidopsis thaliana | ? | - |
? | |
additional information | AtPAO3 catalyzes the sequential conversion/oxidation of spermine to spermidine, and of spermidine to putrescine, thus exhibiting functional homology to the mammalian PAOs, but AtPAO3 does not catalyze the conversion of putrescine back to spermine | Arabidopsis thaliana | ? | - |
? | |
additional information | substrate specificity of AtPAO3, the best substrate is Spd, whereas the N1-acetyl-derivatives of spermine and spermidine are oxidized less efficiently, no activity with diamines agmatine, cadaverine, and putrescine. AtPAO3 does not catalyze the conversion of putrescine back to spermine | Arabidopsis thaliana | ? | - |
? | |
N1-acetylspermidine + O2 + H2O | weak activity | Arabidopsis thaliana | ? | - |
? | |
N1-acetylspermidine + O2 + H2O | - |
Arabidopsis thaliana | putrescine + 3-acetaminopropanal + H2O2 | - |
? | |
N1-acetylspermine + O2 + H2O | weak activity | Arabidopsis thaliana | ? | - |
? | |
N1-acetylspermine + O2 + H2O | - |
Arabidopsis thaliana | spermidine + 3-acetaminopropanal + H2O2 | - |
? | |
spermidine + O2 + H2O | - |
Arabidopsis thaliana | putrescine + 3-aminopropanal + H2O2 | - |
? | |
spermidine + O2 + H2O | best substrate | Arabidopsis thaliana | putrescine + 3-aminopropanal + H2O2 | - |
? | |
spermine + O2 + H2O | - |
Arabidopsis thaliana | spermidine + 3-aminopropanal + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54100, calculated from sequence | Arabidopsis thaliana |
? | x * 54100, AtPAO3, sequence calculation | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AtPAO3 | - |
Arabidopsis thaliana |
PAO | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Arabidopsis thaliana |
37 | - |
at higher temperatures, AtPAO3 is quickly inactivated | Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | 30°C: about 85% of maximal activity, 40°C: about 60% of maximal activity | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
N1-acetylspermine | 37°C, pH 7.0 | Arabidopsis thaliana | |
0.042 | - |
N1-acetylspermidine | 37°C, pH 7.0 | Arabidopsis thaliana | |
0.188 | - |
spermine | 37°C, pH 7.0 | Arabidopsis thaliana | |
0.188 | - |
spermine | pH 7.5, 37°C, recombinant AtPAO3 | Arabidopsis thaliana | |
1 | - |
N1-acetylspermidine | pH 7.5, 37°C, recombinant AtPAO3 | Arabidopsis thaliana | |
1.25 | - |
spermidine | 37°C, pH 7.0 | Arabidopsis thaliana | |
1.25 | - |
spermidine | pH 7.5, 37°C, recombinant AtPAO3 | Arabidopsis thaliana | |
2 | - |
N1-acetylspermine | pH 7.5, 37°C, recombinant AtPAO3 | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
AtPAO3 pH profile | Arabidopsis thaliana |
7 | 8.5 | pH 7: about 95% of maximal activity, pH 8.5: about 55% of maximal activity | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains approximately 0.8 mol of FAD | Arabidopsis thaliana | |
FAD | 0.8 mol FAD per mol of AtPAO3 protein | Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000028 | - |
guazatine | 37°C, pH 7.0 | Arabidopsis thaliana | |
0.000028 | - |
guazatine | pH 7.5, 37°C, recombinant AtPAO3 | Arabidopsis thaliana | |
0.00108 | - |
aminoguanidine | 37°C, pH 7.0 | Arabidopsis thaliana | |
0.0018 | - |
aminoguanidine | pH 7.5, 37°C, recombinant AtPAO3 | Arabidopsis thaliana | |
0.0408 | - |
N8-Acetylspermidine | 37°C, pH 7.0 | Arabidopsis thaliana | |
0.0408 | - |
N8-Acetylspermidine | pH 7.5, 37°C, recombinant AtPAO3 | Arabidopsis thaliana | |
0.0615 | - |
putrescine | 37°C, pH 7.0 | Arabidopsis thaliana | |
0.0615 | - |
putrescine | pH 7.5, 37°C, recombinant AtPAO3 | Arabidopsis thaliana |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Arabidopsis thaliana | calculated from sequence | - |
5.81 |
Arabidopsis thaliana | AtPAO3, sequence calculation | - |
5.81 |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | AtPAO3 mRNA rapidly accumulats in wounded plants 1 h after wounding and returns to almost basal levels 6 h thereafter. Seedlings treated with flagellin 22, a pathogen elicitor that activates the plant basal defense, exhibits AtPAO3 induction after the 6-h time point, with constant increase up to 24 h | up |