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Literature summary for 1.5.3.17 extracted from

  • Moschou, P.N.; Sanmartin, M.; Andriopoulou, A.H.; Rojo, E.; Sanchez-Serrano, J.J.; Roubelakis-Angelakis, K.A.
    Bridging the gap between plant and mammalian polyamine catabolism: a novel peroxisomal polyamine oxidase responsible for a full back-conversion pathway in Arabidopsis (2008), Plant Physiol., 147, 1845-1857.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
AtPAO3, expression as GFP-tagged enzyme in plant cells, expression of AtPAO3 fused to the maltose-binding protein in Escherichia coli strain BL21 in a temperature-dependent manner, overview Arabidopsis thaliana
expression in Escherichia coli Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
aminoguanidine
-
Arabidopsis thaliana
guazatine
-
Arabidopsis thaliana
N8-Acetylspermidine
-
Arabidopsis thaliana
putrescine
-
Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
N1-acetylspermine pH 7.5, 37°C, recombinant AtPAO3 Arabidopsis thaliana
0.042
-
N1-acetylspermidine pH 7.5, 37°C, recombinant AtPAO3 Arabidopsis thaliana
0.204
-
spermidine 37°C, pH 7.0 Arabidopsis thaliana
0.204
-
spermidine pH 7.5, 37°C, recombinant AtPAO3 Arabidopsis thaliana
0.588
-
spermine 37°C, pH 7.0 Arabidopsis thaliana
0.588
-
spermine pH 7.5, 37°C, recombinant AtPAO3 Arabidopsis thaliana
1
-
N1-acetylspermidine 37°C, pH 7.0 Arabidopsis thaliana
2
-
N1-acetylspermine 37°C, pH 7.0 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome isozyme AtPAO3 contains a peroxisomal targeting motif at the C terminus Arabidopsis thaliana 5777
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54100
-
x * 54100, calculated from sequence Arabidopsis thaliana
54100
-
x * 54100, AtPAO3, sequence calculation Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana AtPAO3 catalyzes the sequential conversion/oxidation of spermine to spermidine, and of spermidine to putrescine, thus exhibiting functional homology to the mammalian PAOs, but AtPAO3 does not catalyze the conversion of putrescine back to spermine ?
-
?
spermidine + O2 + H2O Arabidopsis thaliana
-
putrescine + 3-aminopropanal + H2O2
-
?
spermine + O2 + H2O Arabidopsis thaliana
-
spermidine + 3-aminopropanal + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9LYT1
-
-
Arabidopsis thaliana Q9LYT1 isozyme AtPAO3
-

Purification (Commentary)

Purification (Comment) Organism
-
Arabidopsis thaliana
recombinant AtPAO3 fused to the maltose-binding protein from Escherichia coli Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf mostly expressed in the leaves Arabidopsis thaliana
-
leaf most expression of AtPAO3 Arabidopsis thaliana
-
root low expression of AtPAO3 Arabidopsis thaliana
-
seedling
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with agmatine, cadaverine, and putrescine Arabidopsis thaliana ?
-
?
additional information AtPAO3 catalyzes the sequential conversion/oxidation of spermine to spermidine, and of spermidine to putrescine, thus exhibiting functional homology to the mammalian PAOs, but AtPAO3 does not catalyze the conversion of putrescine back to spermine Arabidopsis thaliana ?
-
?
additional information substrate specificity of AtPAO3, the best substrate is Spd, whereas the N1-acetyl-derivatives of spermine and spermidine are oxidized less efficiently, no activity with diamines agmatine, cadaverine, and putrescine. AtPAO3 does not catalyze the conversion of putrescine back to spermine Arabidopsis thaliana ?
-
?
N1-acetylspermidine + O2 + H2O weak activity Arabidopsis thaliana ?
-
?
N1-acetylspermidine + O2 + H2O
-
Arabidopsis thaliana putrescine + 3-acetaminopropanal + H2O2
-
?
N1-acetylspermine + O2 + H2O weak activity Arabidopsis thaliana ?
-
?
N1-acetylspermine + O2 + H2O
-
Arabidopsis thaliana spermidine + 3-acetaminopropanal + H2O2
-
?
spermidine + O2 + H2O
-
Arabidopsis thaliana putrescine + 3-aminopropanal + H2O2
-
?
spermidine + O2 + H2O best substrate Arabidopsis thaliana putrescine + 3-aminopropanal + H2O2
-
?
spermine + O2 + H2O
-
Arabidopsis thaliana spermidine + 3-aminopropanal + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 54100, calculated from sequence Arabidopsis thaliana
? x * 54100, AtPAO3, sequence calculation Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
AtPAO3
-
Arabidopsis thaliana
PAO
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Arabidopsis thaliana
37
-
at higher temperatures, AtPAO3 is quickly inactivated Arabidopsis thaliana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 40 30°C: about 85% of maximal activity, 40°C: about 60% of maximal activity Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
N1-acetylspermine 37°C, pH 7.0 Arabidopsis thaliana
0.042
-
N1-acetylspermidine 37°C, pH 7.0 Arabidopsis thaliana
0.188
-
spermine 37°C, pH 7.0 Arabidopsis thaliana
0.188
-
spermine pH 7.5, 37°C, recombinant AtPAO3 Arabidopsis thaliana
1
-
N1-acetylspermidine pH 7.5, 37°C, recombinant AtPAO3 Arabidopsis thaliana
1.25
-
spermidine 37°C, pH 7.0 Arabidopsis thaliana
1.25
-
spermidine pH 7.5, 37°C, recombinant AtPAO3 Arabidopsis thaliana
2
-
N1-acetylspermine pH 7.5, 37°C, recombinant AtPAO3 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
AtPAO3 pH profile Arabidopsis thaliana
7 8.5 pH 7: about 95% of maximal activity, pH 8.5: about 55% of maximal activity Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
FAD contains approximately 0.8 mol of FAD Arabidopsis thaliana
FAD 0.8 mol FAD per mol of AtPAO3 protein Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000028
-
guazatine 37°C, pH 7.0 Arabidopsis thaliana
0.000028
-
guazatine pH 7.5, 37°C, recombinant AtPAO3 Arabidopsis thaliana
0.00108
-
aminoguanidine 37°C, pH 7.0 Arabidopsis thaliana
0.0018
-
aminoguanidine pH 7.5, 37°C, recombinant AtPAO3 Arabidopsis thaliana
0.0408
-
N8-Acetylspermidine 37°C, pH 7.0 Arabidopsis thaliana
0.0408
-
N8-Acetylspermidine pH 7.5, 37°C, recombinant AtPAO3 Arabidopsis thaliana
0.0615
-
putrescine 37°C, pH 7.0 Arabidopsis thaliana
0.0615
-
putrescine pH 7.5, 37°C, recombinant AtPAO3 Arabidopsis thaliana

pI Value

Organism Comment pI Value Maximum pI Value
Arabidopsis thaliana calculated from sequence
-
5.81
Arabidopsis thaliana AtPAO3, sequence calculation
-
5.81

Expression

Organism Comment Expression
Arabidopsis thaliana AtPAO3 mRNA rapidly accumulats in wounded plants 1 h after wounding and returns to almost basal levels 6 h thereafter. Seedlings treated with flagellin 22, a pathogen elicitor that activates the plant basal defense, exhibits AtPAO3 induction after the 6-h time point, with constant increase up to 24 h up