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Literature summary for 1.5.3.16 extracted from

  • Kim, D.W.; Watanabe, K.; Murayama, C.; Izawa, S.; Niitsu, M.; Michael, A.J.; Berberich, T.; Kusano, T.
    Polyamine oxidase 5 regulates Arabidopsis growth through Thermospermine oxidase activity (2014), Plant Physiol., 165, 1575-1590 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0256
-
spermine 37°C, pH 7.5 Arabidopsis thaliana
0.0787
-
spermine 37°C, pH 6.5 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thermospermine + O2 + H2O Arabidopsis thaliana
-
?
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9SU79
-
-

Source Tissue

Source Tissue Comment Organism Textmining
procambium
-
Arabidopsis thaliana
-
vascular bundle
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
spermine + O2 + H2O at pH 6.5, substrate preference is in the following decreasing order: thermospermine, N1-acetylspermine, norspermine, spermine, spermidine. Spermidine is catabolized at a very low rate. At pH 7.5, substratr preference is in the following decreasing order: spermine, norspermine, N1-acetylspermine, thermospermine, spermidine. Spermidine is catalyzed at a very low rate Arabidopsis thaliana spermidine + 3-aminopropanal + H2O2
-
?
thermospermine + O2 + H2O
-
Arabidopsis thaliana ?
-
?

Synonyms

Synonyms Comment Organism
AtPAO5
-
Arabidopsis thaliana
thermospermine oxidase
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 42 substrate: spermine Arabidopsis thaliana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 25°C: about 55% of maximal activity, 50°C: about 45% of maximal activity, substrate: spermine Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.009
-
spermine 37°C, pH 6.5 Arabidopsis thaliana
0.069
-
spermine 37°C, pH 7.5 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
substrate: spermine Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
7 9 pH 7.0: about 60% of maximal activity, pH 9.0: about 45% of maximal activity, substrate: spermine Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction loss-of-function mutants contain 2-fold higher thermospermine levels and exhibit delayed transition from vegetative to reproductive growth compared with that of wild-type plants Arabidopsis thaliana
physiological function the enzyme regulates thermospermine homeostasis through a thermospermine oxidation pathway Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.115
-
spermine 37°C, pH 6.5 Arabidopsis thaliana
2.7
-
spermine 37°C, pH 7.5 Arabidopsis thaliana