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Literature summary for 1.5.3.16 extracted from
- A putative peroxisomal polyamine oxidase, AtPAO4, is involved in polyamine catabolism in Arabidopsis thaliana (2008), Plant Cell Physiol., 49, 1272-1282.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli as a His-tagged protein | Arabidopsis thaliana |
| expression of His-tagged AtPAO4 in Escherichia coli strain BL21(DE3), transient expression of isozyme AtPAO4 in Arabidopsis thaliana root cell peroxisomes as monomeric red fluorescent protein fusion protein | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a knock-out mutant that has a T-DNA insertion within the ninth exon of the AtPAO4 gene, and of a knock-down mutant Atpao4i with reduced expression by RNAi under control of the CMV 35S promoter. AtPAO4 deficiency induces alterations in the expression of genes related to the drought stress response and flavonoid biosynthesis | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
spermine | 25°C, pH 8 | Arabidopsis thaliana |  |
| 0.23 | - |
spermine | recombinant His-tagged isozyme AtPAO4 | Arabidopsis thaliana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| peroxisome | - |
Arabidopsis thaliana | 5777 | - |
| peroxisome | AtPAO4 is the major isoform in root peroxisomes | Arabidopsis thaliana | 5777 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 55000, SDS-PAGE | Arabidopsis thaliana |
| 55000 | - |
x * 55000, recombinant His-tagged AtPAO4, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Arabidopsis thaliana | AtPAO4 deficiency induces alterations in the expression of genes related to the drought stress response and flavonoid biosynthesis | ? | - |
? | |
| additional information | Arabidopsis thaliana | peroxisomal polyamine oxidase AtPAO4 is involved in the catabolism of polyamines in leaves, overview | ? | - |
? | |
| spermine + O2 + H2O | Arabidopsis thaliana | - |
spermidine + 3-aminopropanal + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q8H191 | - |
- |
| Arabidopsis thaliana | Q8H191 | isozyme AtPAO4 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| flavoprotein | - |
Arabidopsis thaliana |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Arabidopsis thaliana |
| recombinant His-tagged AtPAO4 from Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | greening | Arabidopsis thaliana | - |
| flower | - |
Arabidopsis thaliana | - |
| leaf | - |
Arabidopsis thaliana | - |
| additional information | isozyme AtPAO4 transcript is abundantly expressed in roots, flowers and greening cotyledons, but more poorly expressed in other tissues | Arabidopsis thaliana | - |
| root | - |
Arabidopsis thaliana | - |
| root | AtPAO4 is the major isoform in root peroxisomes | Arabidopsis thaliana | - |
| seedling | - |
Arabidopsis thaliana | - |
| silique | - |
Arabidopsis thaliana | - |
| stem | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | AtPAO4 deficiency induces alterations in the expression of genes related to the drought stress response and flavonoid biosynthesis | Arabidopsis thaliana | ? | - |
? | |
| additional information | no activity with putrescine, spermidine, N1-acetylspermine | Arabidopsis thaliana | ? | - |
? | |
| additional information | peroxisomal polyamine oxidase AtPAO4 is involved in the catabolism of polyamines in leaves, overview | Arabidopsis thaliana | ? | - |
? | |
| spermine + O2 + H2O | - |
Arabidopsis thaliana | spermidine + 3-aminopropanal + H2O2 | - |
? | |
| spermine + O2 + H2O | AtPAO4 specifically catalyzes the conversion of spermine to spermidine under the assay conditions | Arabidopsis thaliana | spermidine + 3-aminopropanal + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, SDS-PAGE | Arabidopsis thaliana |
| ? | x * 55000, recombinant His-tagged AtPAO4, SDS-PAGE | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtPAO4 | - |
Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
spermine | 25°C, pH 8 | Arabidopsis thaliana | |
| 1.4 | - |
spermine | recombinant His-tagged isozyme AtPAO4 | Arabidopsis thaliana | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Arabidopsis thaliana |
| 7.5 | 8.5 | recombinant His-tagged isozyme AtPAO4 with substrate spermine | Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Arabidopsis thaliana | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | AtPAO4 deficiency induces alterations in the expression of genes related to the drought stress response and flavonoid biosynthesis | Arabidopsis thaliana |
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