Cloned (Comment) | Organism |
---|---|
expression of recombinant enzyme | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
K315M | site-directed mutagenesis, the mutant shows altered kinetics compared to the wild-type enzyme | Mus musculus |
K315M | mutation has no effect on the kcat/Kamine profile for spermine. The kred value with N1-acetylspermine is 1.8fold lower in the mutant protein, and the pKa in the k(red)-pH profile with N1-acetylspermine shifts to 7.8. K315 does not play a critical role in amine oxidation by PAO | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,8-diaminooctane | competitive versus the polyamine | Mus musculus | |
N1-acetyl-1,12-diaminododecane | competitive versus the polyamine | Mus musculus | |
N1-acetyl-1,8-diaminooctane | competitive versus the polyamine | Mus musculus | |
N1-acetyl-N3-pentyl-1,3-diaminopropane | competitive versus the polyamine | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics and rapid-reaction kinetics | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N1-acetylspermidine + O2 + H2O | Mus musculus | - |
putrescine + 3-acetaminopropanal + H2O2 | - |
? | |
N1-acetylspermine + O2 + H2O | Mus musculus | - |
spermidine + 3-acetaminopropanal + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Mus musculus | Q8C0L6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Mus musculus |
recombinant enzyme | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N1-acetylspermidine + O2 + H2O | - |
Mus musculus | ? | - |
? | |
N1-acetylspermidine + O2 + H2O | - |
Mus musculus | putrescine + 3-acetaminopropanal + H2O2 | - |
? | |
N1-acetylspermine + O2 + H2O | - |
Mus musculus | ? | - |
? | |
N1-acetylspermine + O2 + H2O | - |
Mus musculus | spermidine + 3-acetaminopropanal + H2O2 | - |
? | |
spermine + O2 + H2O | - |
Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | PAO is a member of the monoamine oxidase family of flavoproteins | Mus musculus |
PAO | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.9 | 10.5 | substrate-dependent | Mus musculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
effects of pH on the steady-state and reductive half-reaction. Kinetics for N1-acetylspermine, N1-acetylspermidine, and spermine kcat/K(amine)-pH profiles are bell-shaped | Mus musculus |
7 | 11 | pH profile, overview | Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics, pH-dependence, overview | Mus musculus |