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Literature summary for 1.5.3.13 extracted from

  • Henderson Pozzi, M.; Gawandi, V.; Fitzpatrick, P.F.
    pH dependence of a mammalian polyamine oxidase: insights into substrate specificity and the role of lysine 315 (2009), Biochemistry, 48, 1508-1516.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of recombinant enzyme Mus musculus

Protein Variants

Protein Variants Comment Organism
K315M site-directed mutagenesis, the mutant shows altered kinetics compared to the wild-type enzyme Mus musculus
K315M mutation has no effect on the kcat/Kamine profile for spermine. The kred value with N1-acetylspermine is 1.8fold lower in the mutant protein, and the pKa in the k(red)-pH profile with N1-acetylspermine shifts to 7.8. K315 does not play a critical role in amine oxidation by PAO Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
1,8-diaminooctane competitive versus the polyamine Mus musculus
N1-acetyl-1,12-diaminododecane competitive versus the polyamine Mus musculus
N1-acetyl-1,8-diaminooctane competitive versus the polyamine Mus musculus
N1-acetyl-N3-pentyl-1,3-diaminopropane competitive versus the polyamine Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics and rapid-reaction kinetics Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N1-acetylspermidine + O2 + H2O Mus musculus
-
putrescine + 3-acetaminopropanal + H2O2
-
?
N1-acetylspermine + O2 + H2O Mus musculus
-
spermidine + 3-acetaminopropanal + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-
Mus musculus Q8C0L6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Mus musculus
recombinant enzyme Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N1-acetylspermidine + O2 + H2O
-
Mus musculus ?
-
?
N1-acetylspermidine + O2 + H2O
-
Mus musculus putrescine + 3-acetaminopropanal + H2O2
-
?
N1-acetylspermine + O2 + H2O
-
Mus musculus ?
-
?
N1-acetylspermine + O2 + H2O
-
Mus musculus spermidine + 3-acetaminopropanal + H2O2
-
?
spermine + O2 + H2O
-
Mus musculus ?
-
?

Synonyms

Synonyms Comment Organism
More PAO is a member of the monoamine oxidase family of flavoproteins Mus musculus
PAO
-
Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.9 10.5 substrate-dependent Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
effects of pH on the steady-state and reductive half-reaction. Kinetics for N1-acetylspermine, N1-acetylspermidine, and spermine kcat/K(amine)-pH profiles are bell-shaped Mus musculus
7 11 pH profile, overview Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics, pH-dependence, overview Mus musculus