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Literature summary for 1.5.3.1 extracted from

  • Liu, H.; Sun, G.; Ma, X.; Sun, L.; Lu, X.; Zhang, P.
    Purification and characterization of a sarcosine oxidase from Bacillus sp. BSD-8 (2010), Chin. J. Biotechnol., 26, 335-340.
    View publication on PubMed

Application

Application Comment Organism
analysis sarcosine oxidases can be applied in enzymatic measuring of creatinine Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
Ag+ strong inhibition Bacillus sp. (in: Bacteria)
Hg2+ strong inhibition Bacillus sp. (in: Bacteria)
additional information no inhibition by Tween 20 and Triton X-100 Bacillus sp. (in: Bacteria)
SDS strong inhibition Bacillus sp. (in: Bacteria)
Tween 80 strong inhibition Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1
-
sarcosine pH 8.5, 60°C Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
x * 51000 Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sarcosine + H2O + O2 Bacillus sp. (in: Bacteria)
-
glycine + formaldehyde + H2O2
-
?
sarcosine + H2O + O2 Bacillus sp. (in: Bacteria) BSD-8
-
glycine + formaldehyde + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
isolated from soil
-
Bacillus sp. (in: Bacteria) BSD-8
-
isolated from soil
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by ammoniumm sulfate fractionation, hydrophobic interaction and anion exchange chromatography, and gel filtration Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sarcosine + H2O + O2
-
Bacillus sp. (in: Bacteria) glycine + formaldehyde + H2O2
-
?
sarcosine + H2O + O2
-
Bacillus sp. (in: Bacteria) BSD-8 glycine + formaldehyde + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 51000 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
purified enzyme, stable at Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 10 purified enzyme, stable at Bacillus sp. (in: Bacteria)

Cofactor

Cofactor Comment Organism Structure
flavin flavoenzyme with noncovalently bound flavin Bacillus sp. (in: Bacteria)