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Literature summary for 1.5.3.1 extracted from

  • Zhao, G.; Bruckner, R.C.; Jorns, M.S.
    Identification of the oxygen activation site in monomeric sarcosine oxidase: role of lys265 in catalysis (2008), Biochemistry, 47, 9124-9135.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
mutant proteins expressed as His-tag fusion proteins in Escherichia coli BL21(DE3) Bacillus sp. (in: Bacteria)

Protein Variants

Protein Variants Comment Organism
K265A only traces of activity Bacillus sp. (in: Bacteria)
K265M only traces of activity Bacillus sp. (in: Bacteria)
K265Q only traces of activity Bacillus sp. (in: Bacteria)
K265R less than 30% of covalently bound FAD, strongly reduced turnover Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
methylthioacetate competitive Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
1 * 44000 Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sarcosine + O2 + H2O Bacillus sp. (in: Bacteria)
-
formaldehyde + glycine + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant proteins using His-tag Bacillus sp. (in: Bacteria)

Reaction

Reaction Comment Organism Reaction ID
sarcosine + H2O + O2 = glycine + formaldehyde + H2O2 mechanism suggested Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sarcosine + O2 + H2O
-
Bacillus sp. (in: Bacteria) formaldehyde + glycine + H2O2
-
?

Subunits

Subunits Comment Organism
monomer 1 * 44000 Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
monomeric sarcosine oxidase
-
Bacillus sp. (in: Bacteria)
MSOX
-
Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0049
-
sarcosine K265Q mutant, pH 8.0, 25°C Bacillus sp. (in: Bacteria)
0.0066
-
sarcosine K265A mutant, pH 8.0, 25°C Bacillus sp. (in: Bacteria)
0.0076
-
sarcosine K265M mutant, pH 8.0, 25°C Bacillus sp. (in: Bacteria)
0.272
-
sarcosine K265R mutant, pH 8.0, 25°C Bacillus sp. (in: Bacteria)
45.8
-
sarcosine native protein, pH 8.0, 25°C Bacillus sp. (in: Bacteria)

Cofactor

Cofactor Comment Organism Structure
FAD covalently bound Bacillus sp. (in: Bacteria)