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Literature summary for 1.5.3.1 extracted from

  • Inouye, Y.; Nishimura, M.; Matsuda, Y.; Hoshika, H.; Iwasaki, H.; Hujimura, K.; Asano, K.; Nakamura, S.
    Purification and characterization of sarcosine oxidase of Streptomyces origin (1987), Chem. Pharm. Bull., 35, 4194-4202.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA not Streptomyces sp.
Hydroxylamine hydrochloride
-
Streptomyces sp.
N-bromosuccinimide
-
Streptomyces sp.
Ni2+
-
Streptomyces sp.
p-chloromercuribenzoate not inhibitory Streptomyces sp.
SDS
-
Streptomyces sp.
Zn2+
-
Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.58
-
N-Methyl-L-leucine
-
Streptomyces sp.
0.91
-
sarcosine
-
Streptomyces sp.
1.6
-
N-Methyl-DL-alanine
-
Streptomyces sp.
6.7
-
N-Methyl-DL-valine
-
Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
gel filtration, SDS-PAGE Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sarcosine + H2O + O2 Streptomyces sp. involved in creatinine catabolism ?
-
?
sarcosine + H2O + O2 Streptomyces sp. KB210-8SY involved in creatinine catabolism ?
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
KB210-8SY
-
Streptomyces sp. KB210-8SY
-
KB210-8SY
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25
-
-
Streptomyces sp.

Storage Stability

Storage Stability Organism
frozen Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-methyl-DL-alanine + O2 + H2O
-
Streptomyces sp. formaldehyde + DL-alanine + H2O2
-
?
N-methyl-DL-alanine + O2 + H2O
-
Streptomyces sp. KB210-8SY formaldehyde + DL-alanine + H2O2
-
?
N-methyl-DL-valine + O2 + H2O
-
Streptomyces sp. formaldehyde + DL-valine + H2O2
-
?
N-methyl-DL-valine + O2 + H2O
-
Streptomyces sp. KB210-8SY formaldehyde + DL-valine + H2O2
-
?
N-methyl-L-leucine + O2 + H2O
-
Streptomyces sp. formaldehyde + L-leucine + H2O2
-
?
N-methyl-L-leucine + O2 + H2O
-
Streptomyces sp. KB210-8SY formaldehyde + L-leucine + H2O2
-
?
sarcosine + H2O + O2 involved in creatinine catabolism Streptomyces sp. ?
-
?
sarcosine + H2O + O2 involved in creatinine catabolism Streptomyces sp. KB210-8SY ?
-
?
sarcosine + O2 + H2O
-
Streptomyces sp. glycine + formaldehyde + H2O2
-
?
sarcosine + O2 + H2O
-
Streptomyces sp. KB210-8SY glycine + formaldehyde + H2O2
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 10 pH 10: about 80% of maximum activity Streptomyces sp.
6 10 pH 6: about 65% of maximum activity Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9
-
Streptomyces sp.