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Literature summary for 1.5.1.9 extracted from

  • Hutzler, J.; Dancis, J.
    Saccharopine cleavage by a dehydrogenase of human liver (1970), Biochim. Biophys. Acta, 206, 205-214.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
hydroxylamine slight Homo sapiens
NH4Cl slight Homo sapiens
p-hydroxymercuribenzoate
-
Homo sapiens
Sodium bisulfite slight Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
NAD+
-
Homo sapiens
0.5
-
saccharopine
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ strong inhibition Homo sapiens
Hg2+ strong inhibition Homo sapiens
Zn2+ strong inhibition Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O Homo sapiens involved in lysine catabolism L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
-20°C, crude enzyme stable for several months, purified enzyme inactivated within 3 weeks Homo sapiens
2°C, 5-10% loss of activity per week, purified enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine, high specificity Homo sapiens L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
r
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O involved in lysine catabolism Homo sapiens L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Homo sapiens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 37 20°C: 35% of activity maximum, 37°C: 85% of activity maximum Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
5 min Homo sapiens
44
-
5 min, 50% activity Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8 9
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7.8 9.7 half maximal activities at pH 7.8 and 9.7 Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.6 9.9 10 min Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens
NADP+ 20fold higher activity with NAD+ than NAPD+ Homo sapiens