Activating Compound | Comment | Organism | Structure |
---|---|---|---|
4'-phosphopantetheinyl transferase | 4'-phosphopantetheinyl transferase activates dehydrogenase catalysis of ALDH1L2 by modifying Ser375 | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) Codon Plus cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S374A | the mutant is activated by 4'-phosphopantetheinyl transferase | Homo sapiens |
S374A/S375A | the mutant is not activated by 4'-phosphopantetheinyl transferase | Homo sapiens |
S375A | the mutant is not activated by 4'-phosphopantetheinyl transferase | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0114 | - |
10-formyltetrahydrofolate | at pH 7.0 and 30°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14500 | - |
4 * 14500, C-terminal domain, SDS-PAGE | Homo sapiens |
58000 | - |
C-terminal domain, gel filtration | Homo sapiens |
100000 | - |
full-length enzyme, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + NADP+ + H2O | Homo sapiens | - |
tetrahydrofolate + CO2 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | S375 is the putative site of enzyme phosphopantetheinylation | Homo sapiens |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + NADP+ + H2O | - |
Homo sapiens | tetrahydrofolate + CO2 + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 14500, C-terminal domain, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
10-fTHF dehydrogenase | - |
Homo sapiens |
ALDH1L2 | - |
Homo sapiens |
mtFDH | mitochondrial isoform | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens |