Cloned (Comment) | Organism |
---|---|
FDH is cloned | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-formyltetrahydrofolate | covalent attachment completely inhibits | Rattus norvegicus | |
tetrahydrofolate | potent, competitive product inhibitor | Rattus norvegicus | |
tetrahydrofolate | - |
Sus scrofa | |
Trypsin | 20 min, 70% inhibition, rapid but incomplete loss of dehydrogenase activity; tryptic digestion inactivates 10-formyltetrahydrofolate dehydrogenase but not hydrolase activity | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
99000 | - |
x * 99000, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + NADP+ + H2O | Sus scrofa | - |
tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydrofolate + NADP+ + H2O | Rattus norvegicus | a major folate-binding protein of liver cytosol, 10-formyltetrahydrofolate polyglutamates are tightly bound in vivo | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydropteroylhexaglutamate + NADP+ + H2O | Sus scrofa | - |
tetrahydropteroylhexaglutamate + CO2 + NADPH | - |
? | |
10-formyltetrahydropteroylpentaglutamate + NADP+ + H2O | Rattus norvegicus | - |
tetrahydropteroylpentaglutamate + CO2 + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
liver | - |
Sus scrofa | - |
Storage Stability | Organism |
---|---|
-20°C, 20% glycerol, over 12 months, no loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + NADP+ + H2O | - |
Sus scrofa | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydrofolate + NADP+ + H2O | - |
Sus scrofa | tetrahydrofolate + CO2 + NADPH + H+ | very tight binding of product, binds its product rather than its substrate | ? | |
10-formyltetrahydrofolate + NADP+ + H2O | 10-formyltetrahydrofolate is associated with enzyme when it is rapidly isolated, after storage for 24 h before separation of the binding proteins there remains none and tetrahydrofolate is the predominant form bound to enzyme because of the hydrolase activity | Rattus norvegicus | tetrahydrofolate + CO2 + NADPH + H+ | very tight binding of product, binds its product rather than its substrate | ? | |
10-formyltetrahydrofolate + NADP+ + H2O | a major folate-binding protein of liver cytosol, 10-formyltetrahydrofolate polyglutamates are tightly bound in vivo | Rattus norvegicus | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydropteroylhexaglutamate + NADP+ + H2O | - |
Sus scrofa | tetrahydropteroylhexaglutamate + CO2 + NADPH | - |
? | |
10-formyltetrahydropteroylpentaglutamate + NADP+ + H2O | - |
Rattus norvegicus | tetrahydropteroylpentaglutamate + CO2 + NADPH | - |
? | |
additional information | - |
Sus scrofa | ? | - |
? | |
additional information | enzyme exhibit additional to 10-formyltetrahydrofolate dehydrogenase/hydrolase activities NADP+-dependent aldehyde dehydrogenase activity with propanal as preferred substrate | Rattus norvegicus | ? | - |
? | |
additional information | hydrolysis at 25% of dehydrogenase activity | Rattus norvegicus | ? | - |
? | |
additional information | 10-formyltetrahydrofolate dehydrogenase/hydrolase activities occur at the same time and are associated with separate active sites | Rattus norvegicus | ? | - |
? | |
additional information | irreversible covalent linkage of 5-formyltetrahydrofolate to enzyme, 2 mol bound per mol of enzyme monomer, 5-formyltetrahydrofolate is not the natural substrate and arises from 10-formyltetrahydrofolate | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 99000, SDS-PAGE | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
10-formyltetrahydrofolate | 10-formyltetrahydrofolate | Rattus norvegicus | |
10-formyltetrahydrofolate | 10-formyltetrahydrofolate | Sus scrofa | |
5-formyltetrahydrofolate | covalent linkage of 5-formyltetrahydrofolate to enzyme, 2 mol bound per mol of enzyme monomer, it is a minor form of the folate coenzymes and arises from 10-formyltetrahydrofolate | Rattus norvegicus | |
folate | 2 mol of folate bound per mol of enzyme monomer, consistent with the presence of two active sites | Rattus norvegicus | |
NADP+ | NADP+-dependent | Rattus norvegicus | |
NADP+ | NADP+-dependent | Sus scrofa | |
tetrahydrofolate | very tight binding | Rattus norvegicus | |
tetrahydrofolate | very tight binding | Sus scrofa |