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Literature summary for 1.5.1.6 extracted from

  • Johlin, F.C.; Swain, E.; Smith, C.; Tephly, T.R.
    Studies on the mechanism of methanol poisoning: purification and comparison of rat and human liver 10-formyltetrahydrofolate dehydrogenase (1989), Mol. Pharmacol., 35, 745-750.
    View publication on PubMed
Search for more literature for 1.5.1.6

Application

Application Comment Organism
medicine formic acidemia: toxicity of methanol in humans is correlated with formate accumulation as a result of low rates of formate oxidation, which is in vivo dependent on enzyme activity and on hepatic tetrahydrofolate levels Homo sapiens

General Stability

General Stability Organism
in general activity is labile Homo sapiens
in general activity is labile Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 10-formyltetrahydrofolate
-
 Rattus norvegicus
0.05
-
 10-formyltetrahydrofolate
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-
cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
96000
-
 x * 96000, SDS-PAGE Homo sapiens
96000
-
 x * 96000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-formyltetrahydrofolate + NADP+ + H2O Rattus norvegicus
-
 tetrahydrofolate + CO2 + NADPH + H+
-
 ?
10-formyltetrahydrofolate + NADP+ + H2O Homo sapiens folate-dependent pathway is the exclusive route of formate metabolism in primates, plays major role in disposition of formate generated from one-carbon moieties of environmental chemicals, drugs, and foods, importance in the toxicity of methanol tetrahydrofolate + CO2 + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
 Sprague-Dawley rats
-

Purification (Commentary)

Purification (Comment) Organism
free of 10-formyltetrahydrofolate hydrolase activity Homo sapiens
free of 10-formyltetrahydrofolate hydrolase activity Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver activity in human liver is 26% of that in rat liver, reduced amount of enzyme in human liver Homo sapiens
-
liver activity in human liver is 26% of that in rat liver, reduced amount of enzyme in human liver Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens
additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + NADP+ + H2O
-
 Homo sapiens tetrahydrofolate + CO2 + NADPH + H+
-
 ?
10-formyltetrahydrofolate + NADP+ + H2O
-
 Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+
-
 ?
10-formyltetrahydrofolate + NADP+ + H2O folate-dependent pathway is the exclusive route of formate metabolism in primates, plays major role in disposition of formate generated from one-carbon moieties of environmental chemicals, drugs, and foods, importance in the toxicity of methanol Homo sapiens tetrahydrofolate + CO2 + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 96000, SDS-PAGE Homo sapiens
? x * 96000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
 assay at room temperature Homo sapiens
additional information
-
 assay at room temperature Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
10-formyltetrahydrofolate 10-formyltetrahydrofolate Homo sapiens
10-formyltetrahydrofolate 10-formyltetrahydrofolate Rattus norvegicus
NADP+
-
 Homo sapiens
NADP+
-
 Rattus norvegicus
tetrahydrofolate
-
 Homo sapiens
tetrahydrofolate
-
 Rattus norvegicus