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Literature summary for 1.5.1.6 extracted from

  • Rios-Orlandi, E.M.; Zarkadas, C.G.; MacKenzie, R.E.
    Formyltetrahydrofolate dehydrogenase-hydrolase from pig liver: simultaneous assay of the activities (1986), Biochim. Biophys. Acta, 871, 24-35.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
tetrahydrofolate very strong product inhibition Sus scrofa
tetrahydropteroylpolyglutamate inhibition increases with increasing length of polyglutamyl chain Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
NADP+ spectrophotometric assay Sus scrofa
0.0006
-
NADP+ fluorimetric assay Sus scrofa
0.0044
-
10-formyltetrahydrofolate racemic, fluorimetric assay Sus scrofa
0.0075
-
10-formyltetrahydrofolate racemic, spectrophotometric assay Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Sus scrofa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
92500
-
4 * 92500, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
10-formyltetrahydrofolate + NADP+ + H2O Sus scrofa major disposal route for excess one-carbon units produced in folate-mediated metabolism tetrahydrofolate + CO2 + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.64
-
-
Sus scrofa

Storage Stability

Storage Stability Organism
-20°C, dialyzed against 0.01 M potassium phosphate buffer, pH 7.3, 40% glycerol, 0.1 M KCl, several weeks, stable Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + NADP+ + H2O specific for NADP+ Sus scrofa tetrahydrofolate + CO2 + NADPH + H+
-
?
10-formyltetrahydrofolate + NADP+ + H2O major disposal route for excess one-carbon units produced in folate-mediated metabolism Sus scrofa tetrahydrofolate + CO2 + NADPH + H+
-
?
10-formyltetrahydropteroylpolyglutamate + NADP+ + H2O
-
Sus scrofa tetrahydropteroylpolyglutamate + CO2 + NADPH + H+
-
?
additional information bifunctional enzyme: 10-formyltetrahydrofolate dehydrogenase separated from hydrolase activity, activities are functions of different active sites and take place simultaneously in presence of NADP+ Sus scrofa ?
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 92500, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
10-formyltetrahydrofolate
-
Sus scrofa

Cofactor

Cofactor Comment Organism Structure
10-formyltetrahydrofolate 10-formyltetrahydrofolate Sus scrofa
NADP+ NADP+-dependent Sus scrofa
tetrahydrofolate
-
Sus scrofa