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Literature summary for 1.4.99.2 extracted from

  • Kondo, H.; Kagotani, K.; Oshima, M.; Ishimoto, M.
    Purification and some properties of taurine dehydrogenase from a bacterium (1973), J. Biochem., 73, 1269-1278.
    View publication on PubMed
Search for more literature for 1.4.99.2

General Stability

General Stability Organism
Stable to freeze-thawing Bacteria

Inhibitors

Inhibitors Comment Organism Structure
2-heptyl-4-hydroxyquinoline N-oxide some inhibition Bacteria
Atebrin some inhibition Bacteria
Isonicotinic acid 2-isopropylhydrazine strong inhibition Bacteria
methylene blue some inhibition Bacteria
Neocuproine some inhibition Bacteria
p-chloromercuribenzoate strong inhibition Bacteria
Phenelzine strong inhibition Bacteria

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.056
-
 phenazine methosulfate
-
 Bacteria
20
-
 taurine
-
 Bacteria

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Bacteria 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
taurine + H2O + acceptor Bacteria acts only with phenazine methosulfate as electron acceptor sulfoacetaldehyde + NH3 + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Bacteria
-
 gram-negative
-

Purification (Commentary)

Purification (Comment) Organism
homogenous, precipitation, chromatography steps Bacteria

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Bacteria
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.48
-
-
 Bacteria

Storage Stability

Storage Stability Organism
-20°C, 1.5 mg protein per ml, 1 month Bacteria

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hypotaurine + H2O + phenazine methosulfate poor substrate Bacteria ? + NH3 + reduced phenazine methosulfate
-
 ?
taurine + H2O + acceptor acts only with phenazine methosulfate as electron acceptor Bacteria sulfoacetaldehyde + NH3 + reduced acceptor
-
 ?
taurine + H2O + phenazine methosulfate only acts with phenazine methosulfate as electron acceptor Bacteria sulfoacetaldehyde + NH3 + reduced phenazine methosulfate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 40% loss of activity in 20 min Bacteria

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
-
 Bacteria