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Literature summary for 1.4.3.7 extracted from
- Purification and properties of D-glutamic acid oxidase from Octopus vulgaris Lam. (1958), Arch. Biochem. Biophys., 77, 336-349.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ethanol | 2fold activation in the presence of ethanol | Octopus vulgaris |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetate | 68% inhibition at 0.1 mM | Octopus vulgaris | |
| o-Iodosobenzoate | 32% inhibition at 0.1 mM | Octopus vulgaris | |
| p-chloromercuribenzoate | 74% inhibition at 0.01 mM | Octopus vulgaris | |
| urethane | 31% inhibition at 10 mM | Octopus vulgaris | |
| Veronal | 60% inhibition at 8 mM | Octopus vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.5 | - |
D-Aspartate | - |
Octopus vulgaris | |
| 8 | - |
D-glutamate | - |
Octopus vulgaris | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-aspartate + H2O + O2 | Octopus vulgaris | 70% higher activity with pure oxygen than in air | oxaloacetate + NH3 + H2O2 | - |
? | |
| D-aspartate + H2O + O2 | Octopus vulgaris | poorer substrate than D-glutamate | oxaloacetate + NH3 + H2O2 | - |
? | |
| D-glutamate + H2O + O2 | Octopus vulgaris | better substrate than D-aspartate | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
| D-glutamate + H2O + O2 | Octopus vulgaris | highly specific, 70% higher activity with pure oxygen than in air | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Octopus vulgaris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, precipitation techniques, calcium phosphate gel treatment | Octopus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hepatopancreas | - |
Octopus vulgaris | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-aspartate + H2O + O2 | 70% higher activity with pure oxygen than in air | Octopus vulgaris | oxaloacetate + NH3 + H2O2 | - |
? | |
| D-aspartate + H2O + O2 | poorer substrate than D-glutamate | Octopus vulgaris | oxaloacetate + NH3 + H2O2 | - |
? | |
| D-glutamate + H2O + O2 | better substrate than D-aspartate | Octopus vulgaris | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
| D-glutamate + H2O + O2 | highly specific, 70% higher activity with pure oxygen than in air | Octopus vulgaris | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | 8.3 | - |
Octopus vulgaris |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | cannot be replaced by FMN | Octopus vulgaris | |
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