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Literature summary for 1.4.3.7 extracted from
- D-Glutamtoxydase aus der Antennendruse des Flusskrebses Orconectes limosus: Reinigung und Charakterisierung (1968), Z. Naturforsch. B, 23, 1508-1511.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| very stable | Faxonius limosus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | 64% inhibition at 1 mM | Faxonius limosus |  |
| L-aspartate | slight inhibition at 100 mM | Faxonius limosus | |
| L-glutamate | slight inhibition at 100 mM | Faxonius limosus | |
| p-chloromercuribenzoate | 95% inhibition at 0.1 mM | Faxonius limosus | |
| succinate | slight inhibition at 1 mM | Faxonius limosus | |
| Veronal | strong inhibition | Faxonius limosus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.4 | - |
D-Aspartate | - |
Faxonius limosus | |
| 15.2 | - |
D-glutamate | - |
Faxonius limosus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-aspartate + H2O + O2 | Faxonius limosus | 20% higher activity with pure oxygen than in air | oxaloacetate + NH3 + H2O2 | - |
? | |
| D-glutamate + H2O + O2 | Faxonius limosus | highly specific, no deamination of other D-amino acids found, 30% higher activity with pure oxygen than in air | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Faxonius limosus | - |
crayfish | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Faxonius limosus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| antennal gland | - |
Faxonius limosus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.23 | - |
- |
Faxonius limosus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -30°C, no loss of activity for several months | Faxonius limosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-aspartate + H2O + O2 | 20% higher activity with pure oxygen than in air | Faxonius limosus | oxaloacetate + NH3 + H2O2 | - |
? | |
| D-glutamate + H2O + O2 | highly specific, no deamination of other D-amino acids found, 30% higher activity with pure oxygen than in air | Faxonius limosus | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 21 | - |
stable for more than 5 h | Faxonius limosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
with D-glutamate | Faxonius limosus |
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Release 2023.1 (January 2023)
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