Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.3.5 extracted from

  • Huang, S.H.; Shi, R.J.; Zhang, J.Y.; Wang, Z.; Huang, L.Q.
    Cloning and characterization of a pyridoxine 5'-phosphate oxidase from silkworm, Bombyx mori (2009), Insect Mol. Biol., 18, 365-371.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
PNPO gene, DNA and amino acid sequence determination and analysis, genomic organization, expression in Escherichia coli strain BL21 (DE3) Bombyx mori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
2 * 29000, recombinant enzyme, SDS-PAGE Bombyx mori
60000
-
recombinant enzyme, native PAGE Bombyx mori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyridoxamine 5'-phosphate + H2O + O2 Bombyx mori
-
pyridoxal 5'-phosphate + NH3 + H2O2
-
?
pyridoxine 5'-phosphate + H2O + O2 Bombyx mori
-
pyridoxal 5'-phosphate + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Bombyx mori Q1PCB0 single copy PNPO gene
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21 (DE3) to homogeneity Bombyx mori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyridoxamine 5'-phosphate + H2O + O2
-
Bombyx mori pyridoxal 5'-phosphate + NH3 + H2O2
-
?
pyridoxine 5'-phosphate + H2O + O2
-
Bombyx mori pyridoxal 5'-phosphate + H2O2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 29000, recombinant enzyme, SDS-PAGE Bombyx mori
More each monomer containing nine antiparallel beta-strands and five alpha-helical segments, secondary structure and three-dimensional structure, homology modelling, overview Bombyx mori

Synonyms

Synonyms Comment Organism
PNPO
-
Bombyx mori
pyridoxine 5'-phosphate oxidase
-
Bombyx mori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bombyx mori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
recombinant enzyme Bombyx mori

pH Range

pH Minimum pH Maximum Comment Organism
7 10.5
-
Bombyx mori