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Literature summary for 1.4.3.5 extracted from

  • Tsuge, H.; Itoh, K.; Ozeki, K.; Ohashi, K.
    Separation and characterization of two pyridoxamine (pyridoxine) 5'-phosphate oxidases from baker's yeast (1982), Agric. Biol. Chem., 46, 1075-1077.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Saccharomyces cerevisiae
0.009
-
pyridoxine 5'-phosphate enzyme species FI which is assumed to be an artefact of the purification procedure Saccharomyces cerevisiae
0.025
-
pyridoxamine 5'-phosphate enzyme species FI which is assumed to be an artefact of the purification procedure Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyridoxamine 5'-phosphate + FMN + H2O + 2 H+ Saccharomyces cerevisiae
-
pyridoxal 5'-phosphate + FMNH2 + NH3
-
?
pyridoxine 5'-phosphate + FMN Saccharomyces cerevisiae
-
pyridoxal 5'-phosphate + FMNH2
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae
-
2 forms: FI and FII
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme to homogeneity by calcium phosphate resin and anion exchange chromatography, and gel filtration, separation of isozymes PMP oxidase and PNP oxidase on calcium phosphate gel Saccharomyces cerevisiae
partial Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.056
-
substrate pyridoxine 5'-phosphate, enzyme species FI which is assumed to be an artefact of the purification procedure Saccharomyces cerevisiae
0.107
-
substrate pyridoxine 5'-phosphate, enzyme species FII Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the yeast contains PMP oxidase and PNP oxidase isozymes, which can be separated by calcium phosphate gel chromatography Saccharomyces cerevisiae ?
-
?
pyridoxamine 5'-phosphate + FMN + H2O + 2 H+
-
Saccharomyces cerevisiae pyridoxal 5'-phosphate + FMNH2 + NH3
-
?
pyridoxamine 5'-phosphate + FMN + H2O + 2 H+ preferred substrate of the PMP oxidase isozyme Saccharomyces cerevisiae pyridoxal 5'-phosphate + FMNH2 + NH3
-
?
pyridoxamine 5'-phosphate + H2O + O2
-
Saccharomyces cerevisiae pyridoxal 5'-phosphate + NH3 + H2O2
-
?
pyridoxine 5'-phosphate + FMN
-
Saccharomyces cerevisiae pyridoxal 5'-phosphate + FMNH2
-
?
pyridoxine 5'-phosphate + FMN preferred substrate of the PNP oxidase isozyme Saccharomyces cerevisiae pyridoxal 5'-phosphate + FMNH2
-
?

Synonyms

Synonyms Comment Organism
PMP oxidase
-
Saccharomyces cerevisiae
PNP oxidase
-
Saccharomyces cerevisiae
pyridoxamine (pyridoxine) 5'-phosphate oxidase
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
FMN
-
Saccharomyces cerevisiae