KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Saccharomyces cerevisiae | |
0.009 | - |
pyridoxine 5'-phosphate | enzyme species FI which is assumed to be an artefact of the purification procedure | Saccharomyces cerevisiae | |
0.025 | - |
pyridoxamine 5'-phosphate | enzyme species FI which is assumed to be an artefact of the purification procedure | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridoxamine 5'-phosphate + FMN + H2O + 2 H+ | Saccharomyces cerevisiae | - |
pyridoxal 5'-phosphate + FMNH2 + NH3 | - |
? | |
pyridoxine 5'-phosphate + FMN | Saccharomyces cerevisiae | - |
pyridoxal 5'-phosphate + FMNH2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae | - |
2 forms: FI and FII | - |
Purification (Comment) | Organism |
---|---|
native enzyme to homogeneity by calcium phosphate resin and anion exchange chromatography, and gel filtration, separation of isozymes PMP oxidase and PNP oxidase on calcium phosphate gel | Saccharomyces cerevisiae |
partial | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.056 | - |
substrate pyridoxine 5'-phosphate, enzyme species FI which is assumed to be an artefact of the purification procedure | Saccharomyces cerevisiae |
0.107 | - |
substrate pyridoxine 5'-phosphate, enzyme species FII | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the yeast contains PMP oxidase and PNP oxidase isozymes, which can be separated by calcium phosphate gel chromatography | Saccharomyces cerevisiae | ? | - |
? | |
pyridoxamine 5'-phosphate + FMN + H2O + 2 H+ | - |
Saccharomyces cerevisiae | pyridoxal 5'-phosphate + FMNH2 + NH3 | - |
? | |
pyridoxamine 5'-phosphate + FMN + H2O + 2 H+ | preferred substrate of the PMP oxidase isozyme | Saccharomyces cerevisiae | pyridoxal 5'-phosphate + FMNH2 + NH3 | - |
? | |
pyridoxamine 5'-phosphate + H2O + O2 | - |
Saccharomyces cerevisiae | pyridoxal 5'-phosphate + NH3 + H2O2 | - |
? | |
pyridoxine 5'-phosphate + FMN | - |
Saccharomyces cerevisiae | pyridoxal 5'-phosphate + FMNH2 | - |
? | |
pyridoxine 5'-phosphate + FMN | preferred substrate of the PNP oxidase isozyme | Saccharomyces cerevisiae | pyridoxal 5'-phosphate + FMNH2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PMP oxidase | - |
Saccharomyces cerevisiae |
PNP oxidase | - |
Saccharomyces cerevisiae |
pyridoxamine (pyridoxine) 5'-phosphate oxidase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Saccharomyces cerevisiae |