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Literature summary for 1.4.3.5 extracted from

  • Merrill, A.H.; Kazarinoff, M.N.; Tsuge, H.; Horiike, K.; McCormick, D.B.
    Pyridoxamine (pyridoxine) 5-phosphate oxidase from rabbit liver (1979), Methods Enzymol., 62, 568-574.
    View publication on PubMed
Search for more literature for 1.4.3.5

General Stability

General Stability Organism
bovine serum albumin stabilizes enzyme in dilute solution Oryctolagus cuniculus
repeated thawing and refreezing: denaturation Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
4-deoxypyridoxine 5'-phosphate
-
 Oryctolagus cuniculus
pyridoxal 5'-phosphate
-
 Oryctolagus cuniculus
pyridoxal 5'-phosphate oxime
-
 Oryctolagus cuniculus
pyridoxaloxime 5'-phosphate
-
 Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 2 * 27000, SDS-PAGE Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyridoxamine 5'-phosphate + H2O + O2 Oryctolagus cuniculus in conjugation with pyridoxal kinase the enzyme is responsible for the formation of the coenzyme pyridoxal 5'-phosphate, from the B6 vitamers pyridoxine and pyridoxamine pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.41
-
-
 Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
-20°C, 4 years stable, shell-frozen apo- and holoenzyme Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyridoxamine 5'-phosphate + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?
pyridoxamine 5'-phosphate + H2O + O2 in conjugation with pyridoxal kinase the enzyme is responsible for the formation of the coenzyme pyridoxal 5'-phosphate, from the B6 vitamers pyridoxine and pyridoxamine Oryctolagus cuniculus pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?
pyridoxine 5'-phosphate + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + H2O2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 27000, SDS-PAGE Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
FAD bound poorly to apoenzyme Oryctolagus cuniculus
FMN binds 1 mol of FMN per mol of dimer Oryctolagus cuniculus
FMN analogs modified at position 2, 3, 7, 8, 8alpha and at the ribityl side chain are all bound by the apoenzyme but less tightly than FMN Oryctolagus cuniculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0018
-
 4'-deoxypyridoxine-5'-phosphate
-
 Oryctolagus cuniculus
0.002
-
 pyridoxal 5'-phosphate oxime
-
 Oryctolagus cuniculus
0.003
-
 pyridoxal 5'-phosphate
-
 Oryctolagus cuniculus