Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ammonium sulfate | short incubation activates isozyme E-II, not E-I | Triticum aestivum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ammonium sulfate | 1.9 M, E II: activation at short incubation time, E I: complete inactivation after 10 h incubation; inactivation of isozyme E-I after 10 h | Triticum aestivum | |
Cd2+ | 0.5 mM, isozyme E I: insensitive, isozyme E II: 44% inhibition; 44% reduced activity of isozyme E-II, no inhibition of isozyme E-I | Triticum aestivum | |
citrate-phosphate buffer | - |
Triticum aestivum | |
Hg2+ | 0.5 mM, isozyme E I: insensitive, isozyme E II: 54% inhibition; 72% reduced activity of isozyme E-II, no inhibition of isozyme E-I | Triticum aestivum | |
Zn2+ | 0.5 mM, isozyme E I: insensitive, isozyme E II: 54% inhibition; 72% reduced activity of isozyme E-II, no inhibition of isozyme E-I | Triticum aestivum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0067 | - |
pyridoxamine 5'-phosphate | isozyme E-II, pH 8.0, 37°C | Triticum aestivum | |
0.008 | - |
pyridoxine 5'-phosphate | isozyme E-II, pH 8.0, 37°C | Triticum aestivum | |
0.02 | - |
pyridoxine 5'-phosphate | isozyme E-I, pH 8.0, 37°C | Triticum aestivum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Triticum aestivum | - |
isozymes: E1 and E2 | - |
Triticum aestivum | - |
two isozymes E-I and E-II | - |
Purification (Comment) | Organism |
---|---|
two isozymes from seedlings, partially by anion exchange and adsorption chromatography | Triticum aestivum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Triticum aestivum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00188 | - |
isozyme EII | Triticum aestivum |
0.00367 | - |
isozyme EI | Triticum aestivum |
0.113 | - |
purified isozyme E-II, substrate pyridoxine 5'-phosphate | Triticum aestivum |
0.45 | - |
purified isozyme E-I, substrate pyridoxine 5'-phosphate | Triticum aestivum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridoxamine 5'-phosphate + FMN + H2O + 2 H+ | 1.6fold preferred substrate of isozyme E-II | Triticum aestivum | pyridoxal 5'-phosphate + FMNH2 + NH3 | - |
? | |
pyridoxamine 5'-phosphate + H2O + O2 | E II uses pyridoxine 5'-phosphate and pyridoxamine 5'-phosphate | Triticum aestivum | pyridoxal 5'-phosphate + NH3 + H2O2 | - |
? | |
pyridoxamine 5'-phosphate + H2O + O2 | isozymes E I uses only pyridoxine 5'-phosphate as substrate | Triticum aestivum | pyridoxal 5'-phosphate + NH3 + H2O2 | - |
? | |
pyridoxine 5'-phosphate + FMN | 9fold preferred substrate of isozyme E-I | Triticum aestivum | pyridoxal 5'-phosphate + FMNH2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pyridoxamine (pyridoxine) 5'-phosphate oxidase | - |
Triticum aestivum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Triticum aestivum |
40 | - |
isozymes EI and EII, rapid decrease above | Triticum aestivum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
rapid decrease in activity above 40°C, both isozymes | Triticum aestivum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Triticum aestivum |
9.5 | - |
both isozymes | Triticum aestivum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.1 | - |
37°C, 30 min, isozyme E I: 85% loss of activity, isozyme E II: 50% loss of activity | Triticum aestivum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Triticum aestivum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Triticum aestivum | the pI values of the isozymes differ from each other | - |
additional information |