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Literature summary for 1.4.3.5 extracted from
- Effects of culture medium on pyridoxine (pyridoxamine) phosphate oxidase activity in wheat callus (1988), Agric. Biol. Chem., 52, 343-348.
No PubMed abstract available
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| ammonium | in culture medium, activates PMP oxidase activity in callus culture by about 30%, no activation of PNP oxidase | Triticum aestivum |  |
| potassium nitrate | in culture medium, activates PNP oxidase activity 2fold in callus culture, no activation of PMP oxidase | Triticum aestivum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyridoxamine 5'-phosphate + FMN + H2O + 2 H+ | Triticum aestivum | - |
pyridoxal 5'-phosphate + FMNH2 + NH3 | - |
? | |
| pyridoxine 5'-phosphate + FMN | Triticum aestivum | - |
pyridoxal 5'-phosphate + FMNH2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| callus culture | cultured from immature embryos, effects of culture medium on enzyme activity in callus cultures | Triticum aestivum | - |
| cell suspension culture | - |
Triticum aestivum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.000065 | - |
activity in suspension of cultered cells, substrate pyridoxamine 5'-phosphate | Triticum aestivum |
| 0.000065 | - |
PMP oxidase activity in cell suspension culture | Triticum aestivum |
| 0.00007 | - |
PMP oxidase activity in callus culture | Triticum aestivum |
| 0.000072 | - |
activity in callus, substrate pyridoxamine 5'-phosphate | Triticum aestivum |
| 0.000097 | - |
activity in cell suspension cultures, substrate pyridoxine 5'-phosphate | Triticum aestivum |
| 0.0001 | - |
activity in callus, substrate pyridoxine 5'-phosphate | Triticum aestivum |
| 0.0001 | - |
PNP oxidase activity in callus culture and in cell suspension culture | Triticum aestivum |
| 0.00012 | - |
activity in seedlings, substrate pyridoxamine 5'-phosphate | Triticum aestivum |
| 0.00012 | - |
PMP oxidase activity in seedlings | Triticum aestivum |
| 0.0005 | - |
activity in seedlings, substrate pyridoxine 5'-phosphate | Triticum aestivum |
| 0.0005 | - |
PNP oxidase activity in seedlings | Triticum aestivum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyridoxamine 5'-phosphate + FMN + H2O + 2 H+ | - |
Triticum aestivum | pyridoxal 5'-phosphate + FMNH2 + NH3 | - |
? | |
| pyridoxamine 5'-phosphate + H2O + O2 | - |
Triticum aestivum | pyridoxal 5'-phosphate + NH3 + H2O2 | - |
? | |
| pyridoxine 5'-phosphate + FMN | - |
Triticum aestivum | pyridoxal 5'-phosphate + FMNH2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PMP oxidase | - |
Triticum aestivum |
| PNP oxidase | - |
Triticum aestivum |
| pyridoxamine phosphate oxidase | - |
Triticum aestivum |
| pyridoxine (pyridoxamine) phosphate oxidase | - |
Triticum aestivum |
| pyridoxine phosphate oxidase | - |
Triticum aestivum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Triticum aestivum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Triticum aestivum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | - |
Triticum aestivum | |
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