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Literature summary for 1.4.3.3 extracted from

  • Gisby, M.F.; Mudd, E.A.; Day, A.
    Growth of transplastomic cells expressing D-amino acid oxidase in chloroplasts is tolerant to D-alanine and inhibited by D-valine (2012), Plant Physiol., 160, 2219-2226.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3)pLysS and in Nicotiana tabacum plastids Schizosaccharomyces pombe

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
x * 35000, SDS-PAGE Schizosaccharomyces pombe
39000
-
x * 39000, calculated from amino acid sequence Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-amino acid + H2O + O2 Schizosaccharomyces pombe
-
2-oxocarboxylate + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-alanine + H2O + O2
-
Schizosaccharomyces pombe pyruvate + NH3 + H2O2
-
?
D-amino acid + H2O + O2
-
Schizosaccharomyces pombe 2-oxocarboxylate + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Schizosaccharomyces pombe
? x * 39000, calculated from amino acid sequence Schizosaccharomyces pombe

Synonyms

Synonyms Comment Organism
D-amino acid oxidase
-
Schizosaccharomyces pombe
DAAO
-
Schizosaccharomyces pombe

Cofactor

Cofactor Comment Organism Structure
FAD
-
Schizosaccharomyces pombe

General Information

General Information Comment Organism
physiological function expression of a D-amino acid oxidase gene from Schizosaccharomyces pombe in tobacco chloroplasts give rise to transplastomic ptdao plants that resemble wild-type plants in growth and appearance. Growth of ptdao cells is tolerant to D-alanine but inhibited by D-valine Schizosaccharomyces pombe