Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)Star cells | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-chlorobenzo(d)isoxazol-3-ol | - |
Rattus norvegicus | |
anthranilate | - |
Rattus norvegicus | |
benzoate | - |
Rattus norvegicus | |
chlorpromazine | FAD-competitive inhibitor | Rattus norvegicus | |
crotonate | - |
Rattus norvegicus | |
sulfite | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
O2 | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
15 | - |
D-tryptophan | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
35 | - |
D-phenylalanine | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
86 | - |
D-proline | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
140 | - |
D-alanine | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
310 | - |
D-serine | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
1 * 40000, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-amino acid + H2O + O2 | Rattus norvegicus | - |
2-oxocarboxylate + NH3 + H2O2 | - |
? | |
additional information | Rattus norvegicus | the enzyme interacts with the mammalian protein modulator pLG72 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-chelate affinity column chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-alanine + H2O + O2 | - |
Rattus norvegicus | pyruvate + NH3 + H2O2 | - |
? | |
D-amino acid + H2O + O2 | - |
Rattus norvegicus | 2-oxocarboxylate + NH3 + H2O2 | - |
? | |
D-phenylalanine + H2O + O2 | - |
Rattus norvegicus | phenylpyruvate + NH3 + H2O2 | - |
? | |
D-proline + H2O + O2 | - |
Rattus norvegicus | 2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
D-serine + H2O + O2 | - |
Rattus norvegicus | 2-oxo-3-hydroxypropionic acid + NH3 + H2O2 | - |
? | |
D-tryptophan + H2O + O2 | - |
Rattus norvegicus | indol-3-pyruvate + NH3 + H2O2 | - |
? | |
additional information | the enzyme interacts with the mammalian protein modulator pLG72 | Rattus norvegicus | ? | - |
? | |
additional information | D-aspartate and glycine are no substrates | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
D-amino acid oxidase | - |
Rattus norvegicus |
DAAO | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.7 | - |
D-tryptophan | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
6.4 | - |
D-serine | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
8.5 | - |
D-phenylalanine | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
27 | - |
D-alanine | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
40 | - |
O2 | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
47 | - |
D-proline | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
benzoate | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
1.6 | - |
anthranilate | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
D-serine | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
0.2 | - |
D-alanine | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
0.24 | - |
D-phenylalanine | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
0.25 | - |
D-tryptophan | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
0.55 | - |
D-proline | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus |