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Literature summary for 1.4.3.3 extracted from

  • Gholizadeh, A.; Kohnehrouz, B.B.
    Molecular cloning and expression in Escherichia coli of an active fused Zea mays L. D-amino acid oxidase (2009), Biochemistry (Moscow), 74, 137-144.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparison, expression of the enzyme fused to the maltose-binding protein in Escherichia coli strain TB1 Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
x * 78000, recombinant maltose-binding protein fusion protein, SDS-PAGE Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-alanine + H2O + O2 Zea mays
-
pyruvate + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Zea mays A0PFJ3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme fused to the maltose-binding protein from Escherichia coli strain TB1 by maltose affinity chromatography Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
leaf from plants grown on D-alanine as nitrogen source Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-alanine + H2O + O2
-
Zea mays pyruvate + NH3 + H2O2
-
?
D-alanine + H2O + O2 recombinant maltose-binding protein-fusion DAAO Zea mays pyruvate + NH3 + H2O2
-
?
D-aspartate + H2O + O2 recombinant maltose-binding protein-fusion DAAO Zea mays ? + NH3 + H2O2
-
?
additional information recombinant maltose-binding protein-fusion DAAO can oxidize D-alanine and D-aspartate, but not D-leucine, D-isoleucine, and D-serine Zea mays ?
-
?

Subunits

Subunits Comment Organism
? x * 78000, recombinant maltose-binding protein fusion protein, SDS-PAGE Zea mays

Synonyms

Synonyms Comment Organism
DAAO
-
Zea mays

Cofactor

Cofactor Comment Organism Structure
FAD dependent on Zea mays