Application | Comment | Organism |
---|---|---|
medicine | human gene therapy | Rhodotorula toruloides |
medicine | human gene therapy | Trigonopsis variabilis |
synthesis | oxidation of cephalosporin C in the two-step formation of 7-aminocephalosporanic acid | Rhodotorula toruloides |
synthesis | oxidation of cephalosporin C in the two-step formation of 7-aminocephalosporanic acid | Trigonopsis variabilis |
Cloned (Comment) | Organism |
---|---|
- |
Rhodotorula toruloides |
expression in Kluyveromyces lactis | Trigonopsis variabilis |
expression in Saccharomyces cerevisiae | Trigonopsis variabilis |
Protein Variants | Comment | Organism |
---|---|---|
Y223F | slower substrate binding than the wild-type | Trigonopsis variabilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
alpha2, 2 * 40000, SDS-PAGE | Trigonopsis variabilis |
79000 | - |
- |
Rhodotorula toruloides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O + O2 | Rhodotorula toruloides | - |
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2 | - |
? | |
cephalosporin C + H2O + O2 | Trigonopsis variabilis | - |
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rhodotorula toruloides | - |
- |
- |
Sus scrofa | - |
- |
- |
Trigonopsis variabilis | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Homo sapiens | - |
kidney | - |
Sus scrofa | - |
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O + O2 | - |
Rhodotorula toruloides | 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2 | - |
? | |
cephalosporin C + H2O + O2 | - |
Trigonopsis variabilis | 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Rhodotorula toruloides |
dimer | alpha2, 2 * 40000, SDS-PAGE | Trigonopsis variabilis |
monomer | - |
Sus scrofa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
D-alanine | - |
Rhodotorula toruloides | |
0.8 | - |
D-alanine | - |
Rhodotorula toruloides | |
4.2 | - |
D-alanine | mutant Y223S | Rhodotorula toruloides | |
10 | - |
D-alanine | - |
Homo sapiens | |
10 | - |
D-alanine | - |
Sus scrofa | |
210 | - |
D-alanine | mutant Y223F | Rhodotorula toruloides | |
345 | - |
D-alanine | - |
Rhodotorula toruloides | |
350 | - |
D-alanine | - |
Rhodotorula toruloides | |
737 | - |
cephalosporin C | - |
Rhodotorula toruloides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Homo sapiens | |
FAD | flavoprotein | Sus scrofa | |
FAD | flavoprotein | Rhodotorula toruloides | |
FAD | flavoprotein | Trigonopsis variabilis |