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Literature summary for 1.4.3.22 extracted from

  • Gludovacz, E.; Maresch, D.; Bonta, M.; Szoelloesi, H.; Furtmueller, P.G.; Weik, R.; Altmann, F.; Limbeck, A.; Borth, N.; Jilma, B.; Boehm, T.
    Characterization of recombinant human diamine oxidase (rhDAO) produced in Chinese Hamster Ovary (CHO) cells (2016), J. Biotechnol., 227, 120-130 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in CHO-K1 cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
putrescine at pH 8.0 and 30°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ one mol of dimeric enzyme is occupied with 1.99 mol copper Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000
-
gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
histamine + H2O + O2 Homo sapiens
-
(imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzylamine + H2O + O2 29% activity compared to putrescine Homo sapiens benzaldehyde + NH3 + H2O2
-
?
histamine + H2O + O2
-
Homo sapiens (imidazol-4-yl)acetaldehyde + NH3 + H2O2
-
?
histamine + H2O + O2 48% activity compared to putrescine Homo sapiens ?
-
?
putrescine + H2O + O2 100% activity Homo sapiens ?
-
?
spermidine + H2O + O2 71% activity compared to putrescine Homo sapiens ?
-
?
spermine + H2O + O2 42% activity compared to putrescine Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 100000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
ABP1
-
Homo sapiens
Amiloride-binding protein
-
Homo sapiens
DAO
-
Homo sapiens
histaminase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5
-
putrescine at pH 8.0 and 30°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
L-topaquinone
-
Homo sapiens