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Literature summary for 1.4.3.20 extracted from

  • Matsui, D.; Asano, Y.
    Heterologous production of L-lysine epsilon-oxidase by directed evolution using a fusion reporter method (2015), Biosci. Biotechnol. Biochem., 79, 1473-1480 .
    View publication on PubMed
Search for more literature for 1.4.3.20

Activating Compound

Activating Compound Comment Organism Structure
NaCl highest enzyme activity is obtained by incubation with 1.6 M NaCl, which is approximately 1.5fold higher than that of the untreated enzyme Marinomonas mediterranea

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Marinomonas mediterranea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
 L-lysine at pH and °C Marinomonas mediterranea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
 gel filtration Marinomonas mediterranea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + O2 + H2O Marinomonas mediterranea
-
 (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?
L-lysine + O2 + H2O Marinomonas mediterranea NBRC 103028
-
 (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Marinomonas mediterranea F2JXJ3
-
-
Marinomonas mediterranea NBRC 103028 F2JXJ3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine + O2 + H2O
-
 Marinomonas mediterranea (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?
L-lysine + O2 + H2O 100% activity Marinomonas mediterranea (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?
L-lysine + O2 + H2O
-
 Marinomonas mediterranea NBRC 103028 (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?
L-lysine + O2 + H2O 100% activity Marinomonas mediterranea NBRC 103028 (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?
L-ornithine + O2 + H2O 15.1% activity compared to L-lysine Marinomonas mediterranea ? + H2O2 + NH3
-
 ?
L-ornithine + O2 + H2O 15.1% activity compared to L-lysine Marinomonas mediterranea NBRC 103028 ? + H2O2 + NH3
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 97000, SDS-PAGE Marinomonas mediterranea

Synonyms

Synonyms Comment Organism
L-lysine epsilon-oxidase
-
 Marinomonas mediterranea
LodA
-
 Marinomonas mediterranea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Marinomonas mediterranea