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Literature summary for 1.4.3.20 extracted from

  • Gomez, D.; Lucas-Elio, P.; Solano, F.; Sanchez-Amat, A.
    Both genes in the Marinomonas mediterranea lodAB operon are required for the expression of the antimicrobial protein lysine oxidase (2010), Mol. Microbiol., 75, 462-473.
    View publication on PubMed
Search for more literature for 1.4.3.20

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli S17-1 (lambdapir) cells Marinomonas mediterranea

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Marinomonas mediterranea
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
 x * 95000, SDS-PAGE, intracellular enzyme is detected as two bands of approximately 95000 and 200000 Da Marinomonas mediterranea
200000
-
 x * 200000, SDS-PAGE, intracellular enzyme is detected as two bands of approximately 95000 and 200000 Da Marinomonas mediterranea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + O2 + H2O Marinomonas mediterranea
-
 2-aminoadipate 6-semialdehyde + H2O2 + NH3
-
 ?
L-lysine + O2 + H2O Marinomonas mediterranea MMB-1
-
 2-aminoadipate 6-semialdehyde + H2O2 + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Marinomonas mediterranea
-
-
-
Marinomonas mediterranea MMB-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Marinomonas mediterranea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine + O2 + H2O
-
 Marinomonas mediterranea 2-aminoadipate 6-semialdehyde + H2O2 + NH3
-
 ?
L-lysine + O2 + H2O
-
 Marinomonas mediterranea MMB-1 2-aminoadipate 6-semialdehyde + H2O2 + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 200000, SDS-PAGE, intracellular enzyme is detected as two bands of approximately 95000 and 200000 Da Marinomonas mediterranea
? x * 95000, SDS-PAGE, intracellular enzyme is detected as two bands of approximately 95000 and 200000 Da Marinomonas mediterranea

Synonyms

Synonyms Comment Organism
Lod
-
 Marinomonas mediterranea
LodA
-
 Marinomonas mediterranea
lysine-epsilon oxidase
-
 Marinomonas mediterranea

Expression

Organism Comment Expression
Marinomonas mediterranea the expression of the lysine-epsilon oxidase activity requires functional copies of both genes lodA and lodB since mutants lacking either lodA or lodB do not show any enzyme activity additional information