Activating Compound | Comment | Organism | Structure |
---|---|---|---|
FAD | activates at 0.002 mM, inhibits at 0.004-0.008 mM | Coprinopsis cinerea |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3) | Coprinopsis cinerea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Coprinopsis cinerea | |
Ca2+ | slight inhibition | Coprinopsis cinerea | |
Co2+ | slight inhibition | Coprinopsis cinerea | |
Cr3+ | slight inhibition | Coprinopsis cinerea | |
CTAB | strong inhibition | Coprinopsis cinerea | |
Cu2+ | complete inhibition | Coprinopsis cinerea | |
DTT | slight inhibition | Coprinopsis cinerea | |
EDTA | - |
Coprinopsis cinerea | |
FAD | activates at 0.002 mM, inhibits at 0.004-0.008 mM | Coprinopsis cinerea | |
Fe2+ | strong inhibition | Coprinopsis cinerea | |
Fe3+ | slight inhibition | Coprinopsis cinerea | |
K+ | - |
Coprinopsis cinerea | |
Li+ | slight inhibition | Coprinopsis cinerea | |
Mg2+ | slight inhibition | Coprinopsis cinerea | |
Mn2+ | slight inhibition | Coprinopsis cinerea | |
Ni2+ | - |
Coprinopsis cinerea | |
SDS | complete inhibition | Coprinopsis cinerea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | activates slightly | Coprinopsis cinerea | |
additional information | no effect by NaCl | Coprinopsis cinerea | |
Sr2+ | activates slightly | Coprinopsis cinerea | |
Triton X-100 | activates slightly | Coprinopsis cinerea | |
Zn2+ | activates slightly | Coprinopsis cinerea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + O2 + H2O | Coprinopsis cinerea | - |
3-phenylpyruvic acid + ammonia + H2O2 | - |
? | |
L-phenylalanine + O2 + H2O | Coprinopsis cinerea ATCC MYA-4618 | - |
3-phenylpyruvic acid + ammonia + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coprinopsis cinerea | A8NBN1 | - |
- |
Coprinopsis cinerea ATCC MYA-4618 | A8NBN1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme 35fold from Escherichia coli strain Rosetta (DE3) by nickel affinity and hydrophbic interaction chromatography | Coprinopsis cinerea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.04 | - |
purified recombinant enzyme, substrate L-phenylalanine, pH 8.5, 45°C | Coprinopsis cinerea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + O2 + H2O | - |
Coprinopsis cinerea | 3-phenylpyruvic acid + ammonia + H2O2 | - |
? | |
L-phenylalanine + O2 + H2O | - |
Coprinopsis cinerea ATCC MYA-4618 | 3-phenylpyruvic acid + ammonia + H2O2 | - |
? | |
additional information | the enzyme specifically catalyzes the oxidation of L-phenylalanine with racemic NULL,L-phenylalanine mixture as substrate, substrate specificity, overview. The enzyme shows low activity with L-tyrosine and L-hhistidine besides L-phenylalanine, which is the preferred substrate | Coprinopsis cinerea | ? | - |
? | |
additional information | the enzyme specifically catalyzes the oxidation of L-phenylalanine with racemic NULL,L-phenylalanine mixture as substrate, substrate specificity, overview. The enzyme shows low activity with L-tyrosine and L-hhistidine besides L-phenylalanine, which is the preferred substrate | Coprinopsis cinerea ATCC MYA-4618 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 87100, recombinant His-tagged enzyme, SDS-PAGE | Coprinopsis cinerea |
Synonyms | Comment | Organism |
---|---|---|
CC-LPOX | - |
Coprinopsis cinerea |
L-phenylalanine oxidase | - |
Coprinopsis cinerea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Coprinopsis cinerea |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | activity range, profile overview | Coprinopsis cinerea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | thermal denaturing half-lives of the enzyme at 40, 45, 50, 55 and 60°C are 1855, 642, 332, 105 and 23 min, respectively | Coprinopsis cinerea |
55 | - |
purified recombinant enzyme, pH 8.5, loss of 50% activity | Coprinopsis cinerea |
55 | - |
purified recombinant enzyme, within pH range 7.0-9.5, stable up to | Coprinopsis cinerea |
65 | - |
purified recombinant enzyme, pH 8.5, inactivation | Coprinopsis cinerea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Coprinopsis cinerea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10.5 | activity range, profile overview | Coprinopsis cinerea |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | purified recombinant enzyme, highly stable at, 55°C, loss of about 50% activity at pH 3.0-6.0, loss of 40% activity at pH 11.0 | Coprinopsis cinerea |