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Literature summary for 1.4.3.2 extracted from

  • Isobe, K.; Sugawara, A.; Domon, H.; Fukuta, Y.; Asano, Y.
    Purification and characterization of an L-amino acid oxidase from Pseudomonas sp. AIU 813 (2012), J. Biosci. Bioeng., 114, 257-261.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
alpha,alpha'-dipyridyl 119% activity at 1 mM (pH 7.0) Pseudomonas sp.
KCN enhances the lysine oxidase activity (177% activity at 1 mM (pH 5.5)) Pseudomonas sp.
N-ethylmaleimide remarkably enhances the lysine oxidase activity (500% activity at 1 mM (pH 7.0)) Pseudomonas sp.
p-chloromercuribenzoic acid remarkably enhances the lysine oxidase activity Pseudomonas sp.
Sodium azide 116% activity at 1 mM (pH 7.0) Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
8-hydroxyquinoline 76% residual activity at 1 mM (pH 5.5) Pseudomonas sp.
Co2+ 67% residual activity at 1 mM (pH 5.5) Pseudomonas sp.
EDTA 80% residual activity at 1 mM (pH 5.5) Pseudomonas sp.
Fe2+ 62% residual activity at 1 mM (pH 5.5) Pseudomonas sp.
Hg2+ complete inhibition at 1 mM (pH 7.0) Pseudomonas sp.
hydrazine 46% residual activity at 1 mM (pH 5.5) Pseudomonas sp.
hydroxylamine 63% residual activity at 1 mM (pH 5.5) Pseudomonas sp.
iodoacetic acid 0.5% residual activity at 1 mM (pH 7.0) Pseudomonas sp.
KCN 70% residual activity at 1 mM (pH 7.0) Pseudomonas sp.
Mg2+ 78% residual activity at 1 mM (pH 5.5) Pseudomonas sp.
Mn2+ 63% residual activity at 1 mM (pH 5.5) Pseudomonas sp.
Ni2+ 55% residual activity at 1 mM (pH 5.5) Pseudomonas sp.
o-phenanthroline 15% residual activity at 1 mM (pH 5.5) Pseudomonas sp.
phenylhydrazine complete inhibition at 1 mM (pH 5.5) Pseudomonas sp.
Semicarbazide 32% residual activity at 1 mM (pH 5.5) Pseudomonas sp.
Zn2+ 24% residual activity at 1 mM (pH 5.5) Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54500
-
2 * 54500, SDS-PAGE Pseudomonas sp.
110000
-
gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-amino acid + H2O + O2 Pseudomonas sp.
-
2-oxocarboxylate + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Toyopearl column chromatography, phenyl Toyopearl column chromatography, and L-lysine-Toyopearl resin column chromatography Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.003
-
cell-free extract, using L-lysine as substrate, at pH 7.0 and 65°C Pseudomonas sp.
1.16
-
after 389fold purification, using L-lysine as substrate, at pH 7.0 and 65°C Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-amino acid + H2O + O2
-
Pseudomonas sp. 2-oxocarboxylate + NH3 + H2O2
-
?
L-arginine + H2O + O2 6% activity compared to L-lysine Pseudomonas sp. 5-carbamidamido-2-oxopentanoate + NH3 + H2O2
-
?
L-lysine + H2O + O2 100% activity Pseudomonas sp. 6-amino-2-oxohexanoate + NH3 + H2O2
-
?
L-ornithine + H2O + O2 31% activity compared to L-lysine Pseudomonas sp. ?
-
?
additional information the enzyme does not oxidize other L-amino acids and D-amino acids besides L-lysine, L-ornithine and L-arginine Pseudomonas sp. ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 54500, SDS-PAGE Pseudomonas sp.

Synonyms

Synonyms Comment Organism
L-amino acid oxidase
-
Pseudomonas sp.
LAAO
-
Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
temperature optimum for for oxidation of L-ornithine Pseudomonas sp.
50 60 temperature optimum for for oxidation of L-arginine Pseudomonas sp.
65
-
temperature optimum for oxidation of L-lysine Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
pH optimum for oxidation of L-lysine Pseudomonas sp.
9
-
pH optimum for oxidation of L-ornithine and L-arginine Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD
-
Pseudomonas sp.

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas sp. isoelectric focusing
-
4.6

Expression

Organism Comment Expression
Pseudomonas sp. the enzyme is remarkably induced by incubation with L-lysine as a nitrogen source up