General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes | Corynebacterium sp. |
KCl stabilizes | Corynebacterium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | substrate inhibition | Corynebacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
L-phenylalanine | - |
Corynebacterium sp. | |
0.04 | - |
L-lysine | - |
Corynebacterium sp. | |
0.05 | - |
cysteine | - |
Corynebacterium sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Corynebacterium sp. | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | 140000 | gel filtration | Corynebacterium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-amino acid + H2O + O2 | Corynebacterium sp. | utilization of amino acids as nitrogen source | 2-oxo acid + NH3 + H2O2 | - |
? | |
L-amino acid + H2O + O2 | Corynebacterium sp. A20 | utilization of amino acids as nitrogen source | 2-oxo acid + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium sp. | - |
A20 | - |
Corynebacterium sp. A20 | - |
A20 | - |
no activity in Bacillus subtilis subsp. subtilis | - |
- |
- |
no activity in Bacillus subtilis subsp. subtilis NCIB 3610 | - |
- |
- |
no activity in Escherichia coli | - |
- |
- |
no activity in Pseudomonas putida | - |
- |
- |
no activity in Pseudomonas putida T1 | - |
- |
- |
Storage Stability | Organism |
---|---|
4°C, 66 mM KCl, 10 mM 2-mercaptoethanol, 1 month, 5-10% loss of activity | Corynebacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + H2O + O2 | - |
Corynebacterium sp. | 2-oxopropanoic acid + NH3 + H2O2 | - |
? | |
L-alpha-aminobutyric acid + H2O + O2 | - |
Corynebacterium sp. | alpha-ketobutyrate + NH3 + H2O2 | - |
? | |
L-amino acid + H2O + O2 | - |
Corynebacterium sp. | 2-oxo acid + NH3 + H2O2 | - |
? | |
L-amino acid + H2O + O2 | utilization of amino acids as nitrogen source | Corynebacterium sp. | 2-oxo acid + NH3 + H2O2 | - |
? | |
L-amino acid + H2O + O2 | - |
Corynebacterium sp. A20 | 2-oxo acid + NH3 + H2O2 | - |
? | |
L-amino acid + H2O + O2 | utilization of amino acids as nitrogen source | Corynebacterium sp. A20 | 2-oxo acid + NH3 + H2O2 | - |
? | |
L-arginine + H2O + O2 | - |
Corynebacterium sp. | 2-oxo-5-guanidinovaleric acid + NH3 + H2O2 | - |
? | |
L-cysteine + H2O + O2 | - |
Corynebacterium sp. | 2-oxo-3-mercaptopropanoic acid + NH3 + H2O2 | - |
? | |
L-histidine + H2O + O2 | - |
Corynebacterium sp. | 3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2 | - |
? | |
L-isoleucine + H2O + O2 | - |
Corynebacterium sp. | alpha-keto-beta-methyl-pentanoic acid + NH3 + H2O2 | - |
? | |
L-leucine + H2O + O2 | - |
Corynebacterium sp. | 4-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
L-lysine + H2O + O2 | - |
Corynebacterium sp. | 6-amino-2-oxohexanoic acid + NH3 + H2O2 | - |
? | |
L-methionine + H2O + O2 | - |
Corynebacterium sp. | 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2 | - |
? | |
L-ornithine + H2O + O2 | - |
Corynebacterium sp. | 2-oxo-5-aminopentanoate + NH3 + H2O2 | - |
? | |
L-phenylalanine + H2O + O2 | - |
Corynebacterium sp. | phenylpyruvate + NH3 + H2O2 | - |
? | |
L-phenylalanine + H2O + O2 | - |
Corynebacterium sp. A20 | phenylpyruvate + NH3 + H2O2 | - |
? | |
L-serine + H2O + O2 | - |
Corynebacterium sp. | 2-oxo-3-hydroxypropionic acid + NH3 + H2O2 | - |
? | |
L-tryptophan + H2O + O2 | - |
Corynebacterium sp. | indole-3-pyruvate + NH3 + H2O2 | - |
? | |
L-tyrosine + H2O + O2 | - |
Corynebacterium sp. | p-hydroxyphenylpyruvate + NH3 + H2O2 | - |
? | |
additional information | not: proline | Corynebacterium sp. | ? | - |
? | |
additional information | not: L-threonine | Corynebacterium sp. | ? | - |
? | |
additional information | not: proline | Corynebacterium sp. A20 | ? | - |
? | |
additional information | not: L-threonine | Corynebacterium sp. A20 | ? | - |
? |